+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of a rapid culture method for tuberculosis diagnosis: a Latin American multi-center study

Evaluation of a rapid culture method for tuberculosis diagnosis: a Latin American multi-center study

International Journal of Tuberculosis and Lung Disease 10(6): 613-619

Tuberculosis (TB) diagnostic laboratories in Latin America. Evaluation of thin-layer agar (TLA) compared to Löwenstein-Jensen (LJ) culture for the diagnosis of TB. Phase II prospective study in six laboratories. Samples included sputum and extra-pulmonary specimens from patients with a clinical diagnosis of TB. Respiratory samples were decontaminated using NaOH/ NALC; all samples were centrifuged, stained with Ziehl-Neelsen for acid-fast bacilli (AFB), cultured on LJ and TLA and identified according to recommended procedures. Sensitivity and likelihood ratios (LR), growth detection time and contamination rate were calculated for both media. A total of 1118 clinical specimens were studied. Cultures detected Mycobacterium tuberculosis in all AFB-positive samples, whereas for AFB-negative specimens LJ detected 3.2% and TLA 4.4%. Sensitivity was 92.6% (95%CI 87.9-95.9) and 84.7% (95%CI 78.8-89.0) for TLA and LJ, respectively. Positive and negative LRs were similar. Contamination was 5.1% for TLA and 3.0% for LJ. Median time to detection of a positive culture was 11.5 days (95%CI 9.3-15.0) for TLA and 30.5 days (95%CI 26.9-39.0) for LJ (P < 0.0001). Difference in the characteristics of the participating laboratories, the disease prevalence and the number and type of specimens processed did not affect the overall performance of TLA as compared to LJ, supporting the robustness of the method and its feasibility in different laboratory settings.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 048993907

Download citation: RISBibTeXText

PMID: 16776447

Related references

A field evaluation of the Hardy TB MODS Kit™ for the rapid phenotypic diagnosis of tuberculosis and multi-drug resistant tuberculosis. Plos one 9(9): E107258, 2014

A multi-center evaluation of Mtd and culture compared to patient diagnosis. Abstracts of the General Meeting of the American Society for Microbiology 98: 358, 1998

Evaluation of a new automated, rapid, colorimetric culture system using solid medium for laboratory diagnosis of tuberculosis and determination of anti-tuberculosis drug susceptibility. International Journal of Tuberculosis and Lung Disease 8(6): 772-777, 2004

Evaluation of a novel biphasic culture medium for recovery of mycobacteria: a multi-center study. Plos one 7(4): E36331, 2012

Hypertension in seven Latin American cities: the Cardiovascular Risk Factor Multiple Evaluation in Latin America (CARMELA) study. Journal of Hypertension 28(1): 24-34, 2010

Evaluation of multiplex polymerase chain reaction utilising multiple targets in Mycobacterium tuberculosis direct test negative but culture positive cases: a potential method for enhancing the diagnosis of tuberculosis. Indian Journal of Medical Microbiology 31(4): 370-373, 2015

Latin American guidelines for the diagnosis and management of drug-resistant tuberculosis. Archivos de Bronconeumologia 44(10): 578-578, 2008

A toolbox for tuberculosis (TB) diagnosis: an Indian multi-centric study (2006-2008); evaluation of serological assays based on PGL-Tb1 and ESAT-6/CFP10 antigens for TB diagnosis. Plos One 9(5): E96367, 2015

Evaluation of the microscopic observational drug susceptibility assay for rapid and efficient diagnosis of multi-drug resistant tuberculosis. Indian Journal of Medical Microbiology 30(1): 64-68, 2012

A multi-site validation in India of the line probe assay for the rapid diagnosis of multi-drug resistant tuberculosis directly from sputum specimens. Plos one 9(2): E88626, 2014

Multi-center and multi-method evaluation of in vitro activities of ceftaroline against S. aureus. Diagnostic Microbiology and Infectious Disease 85(4): 452-458, 2016

Rapid molecular detection of rifampicin resistance facilitates early diagnosis and treatment of multi-drug resistant tuberculosis: case control study. Plos one 3(9): E3173, 2008

The WOMEN study: what is the optimal method for ischemia evaluation in women? A multi-center, prospective, randomized study to establish the optimal method for detection of coronary artery disease (CAD) risk in women at an intermediate-high pretest likelihood of CAD: study design. Journal of Nuclear Cardiology 16(1): 105-112, 2009

Latin american evaluation of the rapid passive hemagglutination reaction for screening chagas disease. Revista do Instituto de Medicina Tropical de Sao Paulo 33(Suppl. 8): S15, 1991

Concomitant pulmonary tuberculosis in hospitalized healthcare-associated pneumonia in a tuberculosis endemic area: a multi-center retrospective study. Plos one 7(5): E36832, 2012