+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of a rapid slide agglutination test for the diagnosis of acute canine leptospirosis



Evaluation of a rapid slide agglutination test for the diagnosis of acute canine leptospirosis



Revista Latinoamericana de Microbiologia 44(3-4): 124-128



The standard method for the diagnostic of leptospirosis is the microscopic agglutination test (MAT). Nevertheless, it is time-consuming and presents risk of infection for laboratory personal. Several attempts to simplify and reduce the time, skill and expense required for serological testing were made. Rapid slide agglutination tests (RSAT) have been used nowadays as a reliable screening test for the detection of acute and recent infections by many laboratories. The purpose of that study was to evaluate a new antigen preparation for the rapid diagnoses of acute canine leptospirosis and to compare it with MAT results in clinically suspect serum samples. Two hundred and thirteen serum samples from dogs of both sexes and different ages were tested and 141 (66.2%) animals were considered as positive by MAT. The most frequent serovar was icterohaemorrhagiae, followed by canicola. RSAT results were very similar to those observed on MAT, with 139 (65.3%) reactive animals. Correlation between RSAT and MAT was positive (0.82) and significant (p < 0.01). Concordance of results was of 93.4% (199/ 213) of the animals correctly diagnosed by RSAT. Considering MAT as the standard test, sensitivity of RSAT was calculated on 94.3% (133/141) and specificity on 91.7% (66/72).

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 048993925

Download citation: RISBibTeXText

PMID: 17061485


Related references

Evaluation of a rapid slide agglutination test for the diagnosis of acute canine leptospirosis. Revista Latinoamericana de Microbiologia 44(3/4): 124-128, 2002

Evaluation of rapid slide agglutination test for the presumptive diagnosis of canine brucellosis. Abstracts of the Annual Meeting of the American Society for Microbiology 77: 37, 1977

Leptospiral rapid slide agglutination and microscopic agglutination test: a comparative study using L interrogans and L. biflexa antigens for the diagnosis of leptospirosis in animals. Arquivos da Escola de Medicina Veterinaria da Universidade Federal da Bahia 19(1): 155-176, 1997

A comparison of the rapid macroscopic slide agglutination test with the microscopic slide agglutination test for leptospirosis. Proceedings Annual Meeting of the United States Animal Health Association 68: 440-444, 1964

A comparison of the rapid macroscopic slide agglutination test with the microscopic agglutination test for leptospirosis. Cornell Veterinarian 57(2): 239-249, 1967

Comparison of agar gel immunodiffusion test, rapid slide agglutination test, microbiological culture and PCR for the diagnosis of canine brucellosis. Research in Veterinary Science 86(1): 22-26, 2009

Improved rapid slide agglutination test for presumptive diagnosis of canine brucellosis. Journal of Clinical Microbiology 15(2): 286-289, 1982

Serological diagnosis of leptospirosis. Comparison of results obtained by a macroscopic slide agglutination test and an agglutination lysis test. Annales de Recherches Veterinaires 11(3): 241-244, 1980

Serological diagnosis of leptospirosis. Comparison of results obtained by macroscopic agglutination in slide test and agglutination-lysis test. Annales de Recherches Veterinaires. Annals of Veterinary Research 11(3): 241-244, 1980

Diagnosis of leptospirosis: a comparision of IgM ELISA, genus specific microscopic agglutination test (MAT) and macroscopic slide agglutination test (MSAT). 2005

Evaluation of a rapid slide agglutination test in the diagnosis of enteric fever. Pasteur Institute of Southern India Coonoor Annual Report of the Director and Scientific Report (1968): 142-146, 1969

Serological diagnosis of leptospirosis by rapid slide agglutination using TR antigen. Bulletin d' Information des Laboratoires des Services Veterinaires (6): 53-55, 1982

Evaluation in Papua New Guinea of a urine coagglutination test and a Widal slide agglutination test for rapid diagnosis of typhoid fever. Transactions of the Royal Society of Tropical Medicine and Hygiene 83(5): 715-717, 1989

Carbon agglutination test for leptospira part 5 serological diagnosis of leptospirosis by means of slide carbon agglutination test. Weishengwu Xuebao 17(2): 154-157, 1977

Evaluation of new slide latex agglutination sla test for the diagnosis of acute candida vaginitis. Abstracts of the Annual Meeting of the American Society for Microbiology 88: 398, 1988