+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of a rat model of experimental autoimmune encephalomyelitis with human MBP as antigen

Evaluation of a rat model of experimental autoimmune encephalomyelitis with human MBP as antigen

Cellular and Molecular Immunology 1(5): 387-391

Experimental autoimmune encephalomyelitis (EAE) is a good model for human multiple sclerosis (MS) research. However, there are some defects in the traditional models. Here, we improved the model by using the human myelin basic protein (MBP) as antigen. EAE was induced by immunization of female Wistar rats with human MBP. Compared with the traditional models, the new model was evaluated by clinical signs to pathological changes. The immune state of the model was assessed by the lymphocyte infiltrative response and levels of TNF-alpha, IFN-gamma, IL-10. It was found that most of rats exhibited tail tone loss and hind-limb paralysis, also there were demyelination, infiltrative lymphocyte foci, "neuronophagia" in the cortex of cerebra and the white matter of spinal cords. PBMCs and spleen lymphocytes were strongly responsive to the stimulation of MBP and PHA. The levels of TNF-alpha and IFN-gamma were altered with the severity of EAE. In the remitting phase, IL-10 was increased significantly. This study demonstrates that the animal model of EAE induced by human MBP bears resemblance to the features of human multiple sclerosis and promises to be a better model than ever before for the study of MS.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 048993933

Download citation: RISBibTeXText

PMID: 16285900

Related references

Interferon in experimental autoimmune encephalomyelitis effects of exogenous interferon on the antigen enhanced adoptive transfer of experimental autoimmune encephalomyelitis. International Archives of Allergy & Applied Immunology 76(4): 302-307, 1985

Parameters associated with the induction of antigen specific tolerance in the adoptive transfer model of experimental autoimmune encephalomyelitis eae in lewis rats. FASEB Journal 5(5): A1359, 1991

Immunomodulation of autoimmunity by linomide: Inhibition of antigen presentation through down regulation of macrophage activity in the model of experimental autoimmune encephalomyelitis. Journal of Neuroimmunology 74(1-2): 102-110, 1997

Evaluation of the Effects of a New Drug on Brain Leukocyte Infiltration in an Experimental Model of Autoimmune Encephalomyelitis. Letters in Drug Design & Discovery 3(3): 138-148, 2006

The experimental autoimmune encephalomyelitis model for proteomic biomarker studies: from rat to human. Clinica Chimica Acta; International Journal of Clinical Chemistry 412(11-12): 812-822, 2011

Evaluation of a radiolabelled PBR ligand in an animal model of experimental autoimmune encephalomyelitis A probe for imaging multiple sclerosis. Journal of Labelled Compounds & Radiopharmaceuticals 46(Suppl. 1): S93, 2003

Pulmonary Administration of Soluble Antigen Arrays Is Superior to Antigen in Treatment of Experimental Autoimmune Encephalomyelitis. Journal of Pharmaceutical Sciences 106(11): 3293-3302, 2017

Regulation of experimental autoimmune encephalomyelitis: inhibition of adoptive experimental autoimmune encephalomyelitis by 'recovery-associated suppressor cells'. Journal of Neuroimmunology 53(2): 123-131, 1994

Expansion by self antigen is necessary for the induction of experimental autoimmune encephalomyelitis by T cells primed with a cross-reactive environmental antigen. Journal of Immunology 161(7): 3307-3314, 1998

Evaluation of the Effects of Sativex (THC BDS: CBD BDS) on Inhibition of Spasticity in a Chronic Relapsing Experimental Allergic Autoimmune Encephalomyelitis: A Model of Multiple Sclerosis. Isrn Neurology 2012: 802649, 2012

Soluble antigen arrays disarm antigen-specific B cells to promote lasting immune tolerance in experimental autoimmune encephalomyelitis. Journal of Autoimmunity 93: 76-88, 2018

Quantitative evaluation of leukocyte infiltration into the spinal cord in a model of experimental autoimmune encephalomyelitis: Statistical-analytical techniques for use in evaluating drugs. International Journal of Immunopathology & Pharmacology 11(3): 117-137, 1998

IL-2/neuroantigen fusion proteins as antigen-specific tolerogens in experimental autoimmune encephalomyelitis (EAE): correlation of T cell-mediated antigen presentation and tolerance induction. Journal of Immunology 178(5): 2835-2843, 2007

Experimental Autoimmune Encephalomyelitis (EAE) Model of Cynomolgus Macaques Induced by Recombinant Human MOG1-125 (rhMOG1-125) Protein and MOG34-56 Peptide. Protein and Peptide Letters 24(12): 1166-1178, 2018

Experimental autoimmune encephalomyelitis: cytokines, effector T cells, and antigen-presenting cells in a prototypical Th1-mediated autoimmune disease. Current Allergy and Asthma Reports 3(1): 86-93, 2003