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Evaluation of a two step AFB cold staining method and simplified concentration technique for diagnosis of pulmonary tuberculosis



Evaluation of a two step AFB cold staining method and simplified concentration technique for diagnosis of pulmonary tuberculosis



Journal of Communicable Diseases 34(4): 276-280



Ziehl Neelsen (Z-N) stain is the commonly used procedure for demonstration for AFB. A Simple cold stain technique and Simplified concentration technique were compared with the conventional methods on 1484 sputum samples over a period of 3 months. A positivity rate of 9.3% was observed on direct microscopy with an increase in the yield by 30% after concentration. It was observed that the simple cold staining and simplified concentration are viable alternatives for demonstration of mycobacteria in busy clinical laboratories.

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Accession: 048994096

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PMID: 14710859


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