EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Free radical formation during splanchnic artery occlusion shock



Free radical formation during splanchnic artery occlusion shock



Journal of Anesthesia 6(4): 414-425



Free radical (FR) formation in the rat intestinal lumen was measured using the spin-trapping technique and electron paramagnetic resonance spectroscopy. Intestinal ischemia was produced by occluding the celiac and the superior mesenteric arteries for 30 min followed by reperfusion. The lumen was filled with a solution of PBN (N-tert-butyl-alpha-phenyl-nitrone) and the intestine was squeezed to enhance the interaction between the PBN solution and the intestinal mucosal cells. Free radicals were produced upon reperfusion, with peaks at 5 and 90 min. Post-ischemic treatment with superoxide dismutase (20 mg.kg(-1)) inhibited the increase of FR production during the second peak by 36%. In a single study in a group of leucocytopenic rats (WBC < 1500/mm(3)), the increase of FR production during the second peak was decreased by 80%. However, these treatments did not inhibit the FR production during the first peak in either group. In contrast, pretreatment with allopurinol (40 and 100 mg.kg(-1) injection at 24 and 3 hours before ischemia, respectively) inhibited the FR production during the first peak by 76%, but did not inhibit during the second peak. The changes in lipid peroxidation in the intestinal mucosa, specific gravity of the intestine and in the hematocrit were correlated to the FR production in the second peak. These results suggest that a major cause of tissue injury after reperfusion in the ischemic intestine may largely be produced by neutrophils.

(PDF emailed within 0-6 h: $19.90)

Accession: 049109978

Download citation: RISBibTeXText

PMID: 15278514

DOI: 10.1007/s0054020060414



Related references

Splanchnic artery occlusion shock in the rats Effects of clevidipine and sodium nitroprusside on splanchnic hemodynamics. FASEB Journal 11(3): A285, 1997

Alterations in splanchnic cyclic nucleotide levels in splanchnic artery occlusion shock and their modification by dexamethasone. European Journal of Pharmacology 37(1): 63-70, 1976

Splanchnic artery occlusion shock: vinblastine-induced leukopenia reduces tumour necrosis factor and thromboxane A2 formation, and increases survival rate. Pharmacological Research 27(1): 61-71, 1993

Comparative evaluation of the acute effects of oxygen free radicals on myocardial contractility in anesthetized dogs with those occurring in the early stages of splanchnic artery occlusion and hemorrhagic shock. Free Radical Biology & Medicine. 17(2): 171-179, 1994

Influence of perflubron on the creation of oxygen free radical products in mesenteric artery occlusion shock. Artificial Cells, Blood Substitutes, and Immobilization Biotechnology 22(4): 1223-1230, 1994

Anti-shock effects of human superoxide dismutase in splanchnic artery occlusion (SAO) shock. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 188(3): 265-271, 1988

Mechanisms of the anti shock effects of human superoxide dismutase in splanchnic artery occlusion sao shock. FASEB Journal 2(5): ABSTRACT 3943, 1988

Use of dopamine in splanchnic artery occlusion shock. Federation Proceedings 36(3): 582, 1977

Actions of dopamine in splanchnic artery occlusion shock. American Journal of Physiology 234(1): H1-H6, 1978

Arachidonic acid in splanchnic artery occlusion shock. American Journal of Physiology 231(1): 112-119, 1976

Amelioration of splanchnic artery occlusion (SAO) shock by cysteine. Advances in Shock Research 1: 261-270, 1978

Therapeutic approaches to splanchnic artery occlusion sao shock in the rat. Pharmacological Research Communications 20(3): 229-230, 1988

Effects of fructose 1,6-diphosphate on splanchnic artery occlusion shock in the rat. Resuscitation 18(2-3): 299-307, 1989

Increased inotropic state during splanchnic artery occlusion shock in the dog. Circulatory Shock 21(2): 97-110, 1987

Protective effects of a novel 21-aminosteroid during splanchnic artery occlusion shock. Circulatory Shock 30(2): 155-164, 1990