EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Genetic diversity of Flavobacterium columnare examined by restriction fragment length polymorphism and sequencing of the 16S ribosomal RNA gene and the 16S-23S rDNA spacer


Genetic diversity of Flavobacterium columnare examined by restriction fragment length polymorphism and sequencing of the 16S ribosomal RNA gene and the 16S-23S rDNA spacer



Molecular and Cellular Probes 19(4): 267-274



ISSN/ISBN: 0890-8508

PMID: 15979275

DOI: 10.1016/j.mcp.2005.04.003

Genetic variability among strains of Flavobacterium columnare, isolated in the United States, was characterized by restriction fragment length polymorphism (RFLP) and phylogenetic analysis based on the sequence of the 16S rRNA gene. Twenty-seven isolates of F. columnare were differentiated into three genotypes. The isolates within the genotypes were further grouped based on RFLP of the 16S-23S rDNA spacer. The first genotype had five strains that were further divided into group A (4 strains) and B (1 strain) while the second genotype had 10 strains that were also further divided into group A (4 strains) and B (6 stains). The third genotype had 12 isolates with no differences in the RFLP patterns of the 16S-23S rDNA spacers. The 16S rRNA gene sequences representing the three identified genotypes were compared to the different published sequences by phylogenetic analysis and the results showed the American genotypes 1, 2 and 3 corresponding to genomovar 1, 2, and 3, respectively, reported by Triyanto and Wakabayashi [Triyanto, Wakabayashi H. Genotyping of strains of Flavobacterium columnare from diseased fishes. Fish Pathol 1999; 34: 65-71]. The study demonstrates a method for RFLP and sequencing of the 16S rRNA gene and the 16-23S rDNA spacer as a useful tool in epidemiological studies of F. columnare.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 049149782

Download citation: RISBibTeXText

Related references

Bacterial diversity in cases of lung infection in cystic fibrosis patients: 16S ribosomal DNA (rDNA) length heterogeneity PCR and 16S rDNA terminal restriction fragment length polymorphism profiling. Journal of Clinical Microbiology 41(8): 3548-3558, 2003

Molecular differentiation of Atractylodes drugs by PCR-restriction fragment length polymorphism and PCR-selective restriction analysis on the 18S-5.8S rDNA intratranscribed spacer 1 gene. Journal of Food and Drug Analysis 5(4): 319-327, 1997

Intraspecific variation in Radopholus similis isolates assessed with restriction fragment length polymorphism and DNA sequencing of the internal transcribed spacer region of the ribosomal RNA cistron. International journal for parasitology 32(2): 199-205, 2002

Restriction fragment length polymorphism of 16S-23S rDNA intergenic spacer of Aeromonas spp. Systematic and Applied Microbiology 27(5): 549-557, 2004

Comparison of two fingerprinting techniques, terminal restriction fragment length polymorphism and automated ribosomal intergenic spacer analysis, for determination of bacterial diversity in aquatic environments. Applied and Environmental Microbiology 72(9): 5982-5989, 2006

Identification of planorbids from Venezuela by polymerase chain reaction amplification and restriction fragment length polymorphism of internal transcriber spacer of the RNA ribosomal gene. Memorias do Instituto Oswaldo Cruz 95(2): 171-177, 2000

Identification of planorbids from Venezuela by polymerase chain reaction amplification and restriction fragment length polymorphism of internal transcriber spacer of the Rna ribosomal gene. Memórias do Instituto Oswaldo Cruz 95(2): 171-177, 2000

A restriction fragment length polymorphism in the 5' non-transcribed spacer of the rDNA of Tetrahymena thermophila inbred strains B and C3. Gene 55(2-3): 169-178, 1987

Molecular epidemiology of a major subgroup of Arthroderma benhamiae isolated in Japan by restriction fragment length polymorphism analysis of the non-transcribed spacer region of ribosomal RNA gene. Japanese Journal of Infectious Diseases 65(3): 233-239, 2012

Restriction fragment length polymorphism of the 5'-region of the bovine ribosomal spacer repeat. Molekuliarnaia Biologiia 26(2): 354-368, 1992

Genetic heterogeneity of Pseudomonas aeruginosa clinical isolates revealed by esterase electrophoretic polymorphism and restriction fragment length polymorphism of the ribosomal RNA gene region. Journal of Medical Microbiology 40(5): 313-322, 1994

A restriction fragment length polymorphism in the 5' non transcribed spacer of the ribosomal dna of tetrahymena thermophila inbred strains b and c3. Gene 55(2-3): 169-178, 1987

Detection of a bovine intergenomic restriction fragment length polymorphism in the untranscribed spacer of the ribosomal repeat. Doklady: biological sciences Akademiia nauk SSSR 313(1-6): 431-433, 1991

Genotypic characterization of Bradyrhizobium strains nodulating endemic woody legumes of the Canary Islands by PCR-restriction fragment length polymorphism analysis of genes encoding 16S rRNA (16S rDNA) and 16S-23S rDNA intergenic spacers, repetitive extragenic palindromic PCR genomic fingerprinting, and partial 16S rDNA sequencing. Applied and Environmental Microbiology 64(6): 2096-2104, 1998

Usefulness of PCR-restriction fragment length polymorphism analysis of the internal transcribed spacer region of rDNA for identification of Anisakis simplex complex. Japanese Journal of Infectious Diseases 59(1): 60-62, 2006