+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Hepatocyte growth factor stimulates proliferation and migration during wound healing of retinal pigment epithelial cells in vitro



Hepatocyte growth factor stimulates proliferation and migration during wound healing of retinal pigment epithelial cells in vitro



Japanese Journal of Ophthalmology 47(3): 268-275



A defect in retinal pigment epithelial (RPE) cells may cause dysfunction of the neural retina, so rapid recovery of differentiated RPE cells is required after RPE injury. We investigated the effect of hepatocyte growth factor (HGF) on wound healing in RPE cells. Confluent monolayers of bovine RPE cells were denuded, and the cells were allowed to recover in the presence or absence of HGF. The effect of HGF on RPE cell proliferation was evaluated by a 3-(4;5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetraz olium assay. In a migration assay, mitomycin C was used to inhibit proliferation, and the number of migrated cells was counted. The signaling pathways involved were examined using inhibitors of mitogen-activated protein kinase (MAPK), phosphatidylinositol-3 (PI3) kinase and protein kinase C pathways. At 80 ng/mL, HGF stimulated the wound closure of RPE monolayers and rendered the restituted cells more epithelioid in shape. HGF at 10 ng/mL stimulated RPE cell migration the most, whereas 80 ng/mL of HGF inhibited migration, but stimulated proliferation the most. In particular, PI3 kinase and MAPK inhibitor inhibited PRE cell migration and proliferation, respectively. HGF stimulated wound closure in cultured RPE cells, and rendered restituted cells epithelioid in shape. HGF may become a therapeutic candidate for RPE wound healing.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 049211788

Download citation: RISBibTeXText

PMID: 12782163

DOI: 10.1016/s0021-5155(03)00003-0


Related references

Hepatocyte growth factor stimulates migration and proliferation during wound healing of retinal pigment epithelial cells in vitro. IOVS 42(4): S810, March 15, 2001

Insulin-like growth factor I stimulates proliferation, migration, and plasminogen activator release by human retinal pigment epithelial cells. Current Eye Research 9(4): 323-335, 1990

Hepatocyte growth factor stimulates proliferation, migration, and lumen formation of human endometrial epithelial cells in vitro. Biology of Reproduction 57(4): 936-942, 1997

Insulin like growth factor i stimulates migration of retinal pigment epithelial cells in culture. Investigative Ophthalmology & Visual Science 29(ABSTR ISSUE): 242, 1988

Tumor necrosis factor-alpha stimulates migration of human retinal pigment epithelial cells in vitro. Investigative Ophthalmology & Visual Science 38(4 PART 1-2): S671, 1997

Basic, not acidic fibroblast growth factor stimulates proliferation of cultured human retinal pigment epithelial cells. Molecular Vision 3: 10, 1998

Hepatocyte growth factor promotes the proliferation of human embryonic stem cell derived retinal pigment epithelial cells. Journal of Cellular Physiology 2018, 2018

Retinal pigment epithelial wound closure in vitro a model to study migration and proliferation. Investigative Ophthalmology & Visual ScienceSuppl.: 304, 1986

Inhibition of retinal pigment epithelial cell migration and proliferation with monoclonal antibodies against the beta-Integrin subunit during wound healing in organ culture. Investigative Ophthalmology & Visual Science 34(9): 2761-2668, 1993

8-chloroadenosine inhibits the growth factor-induced proliferation of retinal pigment epithelial cells in vitro. Chinese Journal of Ophthalmology 34(4): 270-272, 2002

Hepatocyte growth factor delays epithelial wound healing and increases the proliferation of keratocytes. IOVS 42(4): S936, March 15, 2001

Interleukin 1 stimulates migration but not proliferation of pigment epithelial cells in vitro. Investigative Ophthalmology & Visual Science 28(3 SUPPL): 208, 1987

Expression of pigment epithelium-derived factor and thrombospondin-1 regulate proliferation and migration of retinal pigment epithelial cells. Physiological Reports 3(1), 2015

Hepatocyte growth factor stimulated proliferation, migration, and lumen formation of human endometrial epithelial cells in vitro. Biology of Reproduction 57(4): 936-942, 1997

Cross talk between c-Met and epidermal growth factor receptor during retinal pigment epithelial wound healing. Investigative Ophthalmology & Visual Science 48(5): 2242-2248, 2007