High-performance liquid chromatographic stationary phases based on poly (methyloctylsiloxane) immobilized on silica. II. Chromatographic evaluation
Tonhi, E.; Collins, K.E.; Collins, C.H.
Journal of Chromatography. a 948(1-2): 109-119
2002
ISSN/ISBN: 0021-9673
PMID: 12831188
DOI: 10.1016/s0021-9673(02)00006-7
Accession: 049221309
This work describes the chromatographic characterization of stationary phases prepared by deposition of poly(methyloctylsiloxane) (PMOS) on silica followed by immobilization using one of several different processes: thermal treatments (120 or 220 degrees C for 4 h), microwave irradiation (495 W for 15 min), gamma radiation (dose of 80 kGy) or self-immobilization. This evaluation was based on the chromatographic parameters of several test solutes. The stationary phases immobilized at 220 degrees C and which underwent self-immobilization were not appropriate for chromatographic use but the other immobilized phases presented chromatographic performances similar in most respects to a commercial phase (Rainin C8) while the peak characteristics of the basic probe were significantly better with these phases.