+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Human coronavirus-NL63 was detected in specimens from children with acute respiratory infection in Beijing, China



Human coronavirus-NL63 was detected in specimens from children with acute respiratory infection in Beijing, China



Zhonghua Er Ke Za Zhi 44(3): 202-205



A new human coronavirus, HCoV-NL63, was identified recently from two Dutch children with acute respiratory infection (ARI) by two scientists in the Netherlands in 2004. To investigate if this newly discovered virus is associated with acute respiratory infections in pediatric patients in Beijing, tests were developed to detect HCoV-NL63 gene fragments from throat swab and nasopharyngeal aspirates collected from children in outpatient and inpatient departments with ARI in Beijing from Dec. 2003 to Mar. 2004. A total of 245 clinical samples, which were negative either for diagnostic tests of human respiratory syncytial virus, influenza virus A and B, adenovirus, parainfluenza virus 1, 2 and 3 by indirect immunofluorescence assay or human metapneumovirus by RT-PCR, were screened for HCoV-NL63 by nested PCR amplifying gene fragments located on the 1b and 1a genes. Amplicon of PCR from 1a gene of HCoV-NL63 was sequenced and the sequences were compared with those in GenBank nucleotide sequence database. Three (1.2%) out of the 245 samples were positive for HCoV-NL63 by nested-PCR using primers on 1b gene. These three samples also showed positive results on nested PCR in which primers were designed with sequences complementary to 1a gene segments. These positive samples were collected from hospitalized children under 2 years of age with pneumonia, bronchiolitis and bronchitis, respectively. The partial 1a gene sequences from two positive samples (BJ3140 and BJ3787) of HCoV-NL63 showed 100% homology between each other and high homology (98%-99%) with the sequences of 1a gene of HCoV-NL63 reported from different countries in GenBank. Phylogenetic analysis showed that BJ3140 and BJ3787 fell into the same genetic cluster (group 1). These data suggest that some of acute respiratory infections in young children in Beijing area are related to the newly identified HCoV-NL63.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 049251681

Download citation: RISBibTeXText

PMID: 16624059


Related references

Human coronavirus NL63 infection and other coronavirus infections in children hospitalized with acute respiratory disease in Hong Kong, China. Clinical Infectious Diseases 40(12): 1721-1729, 2005

Human coronavirus NL63 is not detected in the respiratory tracts of children with acute Kawasaki disease. Journal of Infectious Diseases 192(10): 1767-1771, 2005

Human coronavirus NL63 in hospitalized children with respiratory infection: a 2-year study from Chongqing, China. Indian Pediatrics 49(10): 825-828, 2012

Analysis of human coronavirus NL63 in 690 children with acute respiratory infection in Shanton. Zhonghua Liu Xing Bing Xue Za Zhi 31(1): 108-109, 2010

Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China. Advances in Virology 2011: 129134, 2011

Community epidemiology of human metapneumovirus, human coronavirus NL63, and other respiratory viruses in healthy preschool-aged children using parent-collected specimens. Pediatrics 120(4): E929-E937, 2007

Prevalence and clinical characteristics of coronavirus NL63 infection in children hospitalized for acute lower respiratory tract infections in Changsha. Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 26(2): 133-135, 2012

Human coronavirus NL63 employs the severe acute respiratory syndrome coronavirus receptor for cellular entry. Proceedings of the National Academy of Sciences of the United States of America 102(22): 7988-7993, 2005

Detection of human coronavirus NL63 and OC43 in children with acute respiratory infections in Niigata, Japan, between 2010 and 2011. Japanese Journal of Infectious Diseases 65(3): 270-272, 2012

Differential downregulation of ACE2 by the spike proteins of severe acute respiratory syndrome coronavirus and human coronavirus NL63. Journal of Virology 84(2): 1198-1205, 2010

The S proteins of human coronavirus NL63 and severe acute respiratory syndrome coronavirus bind overlapping regions of ACE2. Virology 367(2): 367-374, 2007

Serological responses in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63. Clinical and Diagnostic Laboratory Immunology 12(11): 1317-1321, 2005

WU polyomavirus and KI polyomavirus detected in specimens from children with acute respiratory tract infection in China. Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 22(1): 21-23, 2008

Detection of human coronavirus NL63, human metapneumovirus and respiratory syncytial virus in children with respiratory tract infections in south-west Sweden. Clinical Microbiology and Infection 12(11): 1089-1096, 2006

Human coronavirus-NL63 infection is not associated with acute Kawasaki disease. Advances in Experimental Medicine and Biology 581: 523-526, 2006