EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Identification of the interface between cGMP-dependent protein kinase Ibeta and its interaction partners TFII-I and IRAG reveals a common interaction motif


Identification of the interface between cGMP-dependent protein kinase Ibeta and its interaction partners TFII-I and IRAG reveals a common interaction motif



Journal of Biological Chemistry 280(46): 38211-38218



ISSN/ISBN: 0021-9258

PMID: 16166082

DOI: 10.1074/jbc.m507021200

Protein-protein interactions have emerged as an important mechanism providing for specificity in cellular signal transduction. Two splice variants of type I cGMP-dependent protein kinase (PKG Ialpha and Ibeta) differ only in their N-terminal approximately 100 amino acids, which mediate binding to different target proteins. PKG Ibeta, but not Ialpha, binds to the general transcriptional regulator TFII-I and the inositol 1,4,5-trisphosphate receptor-associated PKG substrate IRAG. Using a combination of site-directed mutagenesis and in vitro binding assays, we identified a group of acidic amino acids in the N-terminal leucine zipper dimerization domain of PKG Ibeta required for its binding to both TFII-I and IRAG. Small clusters of basic amino acids in possible alpha-helical regions in TFII-I and IRAG were found to mediate their interaction with PKG Ibeta. Mutation of two negatively charged residues in the PKG Ibeta leucine zipper (D26K/E31R) to positively charged residues, found in corresponding positions in PKG Ialpha, completely abrogated binding to TFII-I and IRAG without disrupting PKG dimerization. Mutation of specific basic residues in TFII-I or IRAG abolished binding of the full-length proteins to PKG Ibeta in intact cells. Based on these results, we propose a model for specific PKG Ibeta interaction with target proteins.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 049278897

Download citation: RISBibTeXText

Related references

Identification of the Interface between cGMP-dependent Protein Kinase I and Its Interaction Partners TFII-I and IRAG Reveals a Common Interaction Motif. Journal of Biological Chemistry 284(6): 38211-38218, 2005

Molecular determinants of the interaction between the inositol 1,4,5-trisphosphate receptor-associated cGMP kinase substrate (IRAG) and cGMP kinase Ibeta. Journal of Biological Chemistry 276(26): 24153-24159, 2001

CGMP-dependent protein kinase I beta physically and functionally interacts with the transcriptional regulator TFII-I. Journal of Biological Chemistry 277(35): 32003-32014, 2002

Regulation of intracellular calcium by a signalling complex of IRAG, IP3 receptor and cGMP kinase Ibeta. Nature 404(6774): 197-201, 2000

Dimerization of cGMP-dependent protein kinase Ibeta is mediated by an extensive amino-terminal leucine zipper motif, and dimerization modulates enzyme function. Journal of Biological Chemistry 278(50): 50070-50079, 2003

Fast and slow cyclic nucleotide-dissociation sites in cAMP-dependent protein kinase are transposed in type Ibeta cGMP-dependent protein kinase. Journal of Biological Chemistry 271(29): 17570-17575, 1996

Autophosphorylation of type Ibeta cGMP-dependent protein kinase increases basal catalytic activity and enhances allosteric activation by cGMP or cAMP. Journal of Biological Chemistry 271(34): 20756-20762, 1996

Isolated regulatory domains of cGMP-dependent protein kinase Ialpha and Ibeta retain dimerization and native cGMP-binding properties and undergo isoform-specific conformational changes. Journal of Biological Chemistry 281(11): 6977-6984, 2006

cGMP stimulation of cystic fibrosis transmembrane conductance regulator Cl- channels co-expressed with cGMP-dependent protein kinase type II but not type Ibeta. Journal of Biological Chemistry 272(7): 4195-4200, 1997

CGMP-dependent protein kinase anchoring by IRAG regulates its nuclear translocation and transcriptional activity. Cellular Signalling 20(7): 1392-1399, 2008

Expression and site specific phosphorylation of cysteine rich protein by cGMP-dependent kinase Ibeta. Gastroenterology 118(4 Suppl 2 Pt 1): A88, 2000

Specific domain of cGMP-dependent protein kinase Ibeta but not Ialpha functions as a transcriptional activator in yeast. Iubmb Life 49(1): 17-22, 2000

A novel interaction of cGMP-dependent protein kinase I with troponin T. Journal of Biological Chemistry 274(52): 37429-37434, 1999

CGMP-dependent protein kinase Ib physically and functionally interacts with the transcriptional regulator TFII-I. The Journal of Biological Chemistry 277(35): 003-14, 2002

Trafficking of cGMP-dependent protein kinase II via interaction with Rab11. Biochemical and Biophysical Research Communications 374(3): 522-526, 2008