EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Keratins modulate colonocyte electrolyte transport via protein mistargeting


Journal of Cell Biology 164(6): 911-921
Keratins modulate colonocyte electrolyte transport via protein mistargeting
The function of intestinal keratins is unknown, although keratin 8 (K8)-null mice develop colitis, hyperplasia, diarrhea, and mistarget jejunal apical markers. We quantified the diarrhea in K8-null stool and examined its physiologic basis. Isolated crypt-units from K8-null and wild-type mice have similar viability. K8-null distal colon has normal tight junction permeability and paracellular transport but shows decreased short circuit current and net Na absorption associated with net Cl secretion, blunted intracellular Cl/HCO3-dependent pH regulation, hyperproliferation and enlarged goblet cells, partial loss of the membrane-proximal markers H,K-ATPase-b and F-actin, increased and redistributed basolateral anion exchanger AE1/2 protein, and redistributed Na-transporter ENaC-c. Diarrhea and protein mistargeting are observed 1-2 d after birth while hyperproliferation/inflammation occurs later. The AE1/2 changes and altered intracellular pH regulation likely account, at least in part, for the ion transport defects and hyperproliferation. Therefore, colonic keratins have a novel function in regulating electrolyte transport, likely by targeting ion transporters to their cellular compartments. Reprinted by permission of the publisher.

(PDF same-day service: $19.90)

Accession: 049435350

PMID: 15007064

DOI: 10.1083/jcb.200308103



Related references

Keratins modulate colonocyte electrolyte transport. Molecular Biology of the Cell 12(Supplement): 57a, 2001

Keratins are potential modulators of colonocyte electrolyte transport. Gastroenterology 120(5 Supplement 1): A 42, April, 2001

Study of the keratins of wool L Isolation of a new protein rich in cystine and proline by peptic hydrolysis of reduced and reconstructed keratins. 1964

The place of short-chain fatty acids in colonocyte metabolism in health and ulcerative colitis The impaired colonocyte barrier. Journal of Pediatric Gastroenterology & Nutrition 21(2): 130-136, 1995

The alpha-2, alpha-5, and beta-1 integrin subunits modulate human Caco-2 colonocyte proliferation and differentiation on a laminin matrix. Molecular Biology of the Cell 6(SUPPL ): 279A, 1995

Keratins modulate hepatic cell adhesion, size and G1/S transition. Experimental Cell Research 313(1): 179-194, 2006

The dispersion of keratins II Studies on the dispersion of keratins by reduction in neutral solutions of protein denaturants. 1943

Wortmannin causes mistargeting of procathepsin D. Evidence for the involvement of a phosphatidylinositol 3-kinase in vesicular transport to lysosomes. The Journal of Cell Biology 130(4): 7-805, 1995

Wortmannin Causes Mistargeting of Procathepsin D: Evidence for the Improvement of a Phosphatidylinositol 3-Kinase in Vesicular Transport to Lysosomes. Journal of Cell Biology 130(4): 797-805, 1995

Characteristics of glucose transport by human and pig colonocyte basolateral membrane. Journal of Physiology (Cambridge) 452: 326P, 1992