EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Keratins modulate colonocyte electrolyte transport via protein mistargeting


, : Keratins modulate colonocyte electrolyte transport via protein mistargeting. Journal of Cell Biology 164(6): 911-921

The function of intestinal keratins is unknown, although keratin 8 (K8)-null mice develop colitis, hyperplasia, diarrhea, and mistarget jejunal apical markers. We quantified the diarrhea in K8-null stool and examined its physiologic basis. Isolated crypt-units from K8-null and wild-type mice have similar viability. K8-null distal colon has normal tight junction permeability and paracellular transport but shows decreased short circuit current and net Na absorption associated with net Cl secretion, blunted intracellular Cl/HCO3-dependent pH regulation, hyperproliferation and enlarged goblet cells, partial loss of the membrane-proximal markers H,K-ATPase-b and F-actin, increased and redistributed basolateral anion exchanger AE1/2 protein, and redistributed Na-transporter ENaC-c. Diarrhea and protein mistargeting are observed 1-2 d after birth while hyperproliferation/inflammation occurs later. The AE1/2 changes and altered intracellular pH regulation likely account, at least in part, for the ion transport defects and hyperproliferation. Therefore, colonic keratins have a novel function in regulating electrolyte transport, likely by targeting ion transporters to their cellular compartments. Reprinted by permission of the publisher.


Accession: 049435350

PMID: 15007064

DOI: 10.1083/jcb.200308103

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Toivola, D.M.; Krishnan, S.; Knickelbein, R.G.; Binder, H.J.; Singh, S.; Omary, M.B.shr, 2001: Keratins modulate colonocyte electrolyte transport. Molecular Biology of the Cell 12(Supplement): 57a, Nov

Krishnan, S.; Toivola, D.M.; Knickelbein, R.G.; Binder, H.J.; Singh, S.; Omary, M.B.shr, 2001: Keratins are potential modulators of colonocyte electrolyte transport. Gastroenterology 120(5 Supplement 1): A 42, April

Anonymous, 1964: Study of the keratins of wool L Isolation of a new protein rich in cystine and proline by peptic hydrolysis of reduced and reconstructed keratins

Roediger, W.E.W., 1995: The place of short-chain fatty acids in colonocyte metabolism in health and ulcerative colitis The impaired colonocyte barrier. Journal of Pediatric Gastroenterology & Nutrition 21(2): 130-136

Emenaker, N.J.; Basson, M.D., 1995: The alpha-2, alpha-5, and beta-1 integrin subunits modulate human Caco-2 colonocyte proliferation and differentiation on a laminin matrix. Molecular Biology of the Cell 6(SUPPL ): 279A

Galarneau, L.; Loranger, A.; Gilbert, Séphane.; Marceau, N., 2006: Keratins modulate hepatic cell adhesion, size and G1/S transition. Keratins (Ks) are the intermediate filament (IF) proteins of epithelial cells. Hepatocyte IFs are made solely of keratins 8 and 18 (K8/K18), the hallmark of all simple epithelia. While K8/K18 are essential for maintaining structural integrity, th...

Anonymous, 1943: The dispersion of keratins II Studies on the dispersion of keratins by reduction in neutral solutions of protein denaturants

Davidson, H.W., 1995: Wortmannin causes mistargeting of procathepsin D. evidence for the involvement of a phosphatidylinositol 3-kinase in vesicular transport to lysosomes. At present little is known of the biochemical machinery controlling transport of newly synthesized lysosomal hydrolases from the trans-Golgi network (TGN) to endosomes. The demonstration that Vps34p (a protein required for targeting soluble hydrol...

Davidson, Howard W., 1995: Wortmannin causes mistargeting of procathepsin D. Evidence for the involvement of a phosphatidylinositol 3-kinase in vesicular transport to lysosomes. At present little is known of the biochemical machinery controlling transport of newly synthesized lysosomal hydrolases from the trans-Golgi network (TGN) to endosomes. The demonstration that Vps34p (a protein required for targeting soluble hydrol...

Davidson, Howard, W., 1995: Wortmannin Causes Mistargeting of Procathepsin D: Evidence for the Improvement of a Phosphatidylinositol 3-Kinase in Vesicular Transport to Lysosomes. At present little is known of the biochemical machinery controlling transport of newly synthesized lysosomal hydrolases from the trans-Golgi network (TGN) to endosomes. The demonstration that Vps34p (a protein required for targeting soluble hydrol...