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Loss of phytochrome photoreversibility in vitro: I. Extraction and partial purification of killer



Loss of phytochrome photoreversibility in vitro: I. Extraction and partial purification of killer



Plant Physiology 55(2): 386-389



Crude pea (Pisum sativum L. var. Alaska) phytochrome extracts contain a substance, "Killer," which interacts with the far red-absorbing form of phytochrome causing a net loss of spectrophotometrically detectable phytochrome in vitro. Killer is absent from crude extracts of Avena phytochrome, is separable from pea phytochrome by gel filtration, and is alcohol-extractable from etiolated pea seedlings. Killer activity in alcohol extracts behaved, during partial purification, in a manner identical to that derived from pea phytochrome preparations. The mass extraction and partial purification of Killer are described.

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Accession: 049495347

Download citation: RISBibTeXText

PMID: 16659088

DOI: 10.1104/pp.55.2.386


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