EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Multicenter evaluation of a new, automated enzyme-linked immunoassay for detection of human immunodeficiency virus-specific antibodies and antigen



Multicenter evaluation of a new, automated enzyme-linked immunoassay for detection of human immunodeficiency virus-specific antibodies and antigen



Journal of Clinical Microbiology 42(1): 21-29



A collaborative multicenter study was conducted to evaluate the sensitivity, specificity, and precision of a three-step, fully automated, qualitative microparticle-based enzyme-linked immunoassay (AxSYM HIV Ag/Ab Combo; Abbott Laboratories), designed to simultaneously detect (i). antibodies against human immunodeficiency virus type 1 (HIV-1) and/or type 2 (HIV-2) and (ii). HIV p24 antigen. A significant reduction in the HIV seroconversion window was achieved by combining detection of HIV antibodies and antigen into a single assay format. For 22 selected, commercial HIV seroconversion panels, the mean time of detection with the combined-format HIV antigen-antibody assay was reduced by 6.15 days compared to that with a similar third-generation single-format HIV antibody assay. The quantitative sensitivity of the combination assay for the p24 antigen (17.5 pg/ml by use of the p24 quantitative panel VIH SFTS96') was nearly equivalent to that of single-format antigen tests. The combination assay demonstrated sensitive (100%) detection of anti-HIV immunoglobulin in specimens from individuals in CDC stages A, B, and C and from individuals infected with different HIV-1 group M subtypes, group O, or HIV-2. The apparent specificity for hospitalized patients (n = 1938) was 99.90%. In a random population of 7900 volunteer blood donors, the specificity (99.87%) was comparable to that of a third-generation single-format HIV antibody assay (99.92%) on the same donor specimens. In addition, the combination assay was robust to potential interfering specimens. The precision of the combination was high, with intra- and interrun variances of

(PDF emailed within 0-6 h: $19.90)

Accession: 049634432

Download citation: RISBibTeXText

PMID: 14715727

DOI: 10.1128/jcm.42.1.21-29.2004



Related references

Evaluation of new fourth-generation human immunodeficiency virus antigen and antibody detection assay with enzyme-linked fluorescent immunoassay. Kansenshogaku Zasshi. Journal of the Japanese Association for Infectious Diseases 81(5): 562-572, 2007

Use of an acute seroconversion panel to evaluate a third-generation enzyme-linked immunoassay for detection of human immunodeficiency virus-specific antibodies relative to multiple other assays. Journal of Clinical Microbiology 44(5): 1856-1858, 2006

Multicenter evaluation of new double-antigen sandwich enzyme immunoassay for measurement of anti-human immunodeficiency virus type 1 and type 2 antibodies. Journal of Clinical Microbiology 34(3): 634-637, 1996

Development of an automated enzyme immunoassay for the detection and quantitation of human immunodeficiency virus hiv antigen. Abstracts of the General Meeting of the American Society for Microbiology 91: 335, 1991

Detection of human immunodeficiency virus (HIV) type 1 antibodies by new automated microparticle enzyme immunoassay for HIV types 1 and 2. Journal of Clinical Microbiology 33(9): 2338-2341, 1995

An automated novel enzyme immunoassay for detection of antibodies to human immunodeficiency virus hiv 1 2 in donor serum and plasma. Abstracts of the General Meeting of the American Society for Microbiology 92: 530, 1992

Enzyme immunoassay for detection of human immunodeficiency virus-specific immunoglobulin A antibodies. Journal of Clinical Microbiology 31(3): 681-684, 1993

Multicenter evaluation of a new rapid automated human immunodeficiency virus antigen detection assay. Journal of Virological Methods 78(1-2): 61-70, March, 1999

Performance of an automated immunoassay for the detection of antibodies to human immunodeficiency virus from a multi-center evaluation. Transfusion (Bethesda) 39(10 SUPPL ): 72S, 1999

More reliable diagnosis of infection with human immunodeficiency virus type 1 (HIV-1) by detection of antibody IgGs to pol and gag proteins of HIV-1 and p24 antigen of HIV-1 in urine, saliva, and/or serum with highly sensitive and specific enzyme immunoassay (immune complex transfer enzyme immunoassay): A review. Journal of Clinical Laboratory Analysis 11(5): 267-286, 1997

Validation of a modified commercial enzyme-linked immunoassay for detection of human immunodeficiency virus type 1 immunoglobulin G antibodies in saliva. Clinical and Diagnostic Laboratory Immunology 8(2): 346-348, 2001

Detection of Epstein-Barr virus-specific antibodies by an automated enzyme immunoassay: Performance evaluation and cost analysis. Diagnostic Microbiology and Infectious Disease 31(4): 549-554, 1998

Multicenter evaluation of a new automated fourth-generation human immunodeficiency virus screening assay with a sensitive antigen detection module and high specificity. Journal of Clinical Microbiology 40(6): 1938-1946, 2002

Evaluation of enzyme-linked immunoassay systems for detection of human immunodeficiency virus type 1 antibody from filter paper disks impregnated with whole blood. Journal of Clinical Microbiology 28(4): 808-810, 1990

Detection of anti-human immunodeficiency virus type 1 (HIV-1) immunoglobulin G in urine by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) with recombinant reverse transcriptase as an antigen. Journal of Clinical Microbiology 32(3): 819-822, 1994