+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

P-glycoprotein is not involved in pathway of anti-Fas/Fas-induced apoptosis in KBv200 cells

P-glycoprotein is not involved in pathway of anti-Fas/Fas-induced apoptosis in KBv200 cells

World Journal of Gastroenterology 11(23): 3544-3548

To verify whether P-glycoprotein (P-gp) could induce cell resistance to apoptosis by inhibiting caspase-8 and caspase-3. Human KB cells, either drug-sensitive or with multidrug resistance (MDR) phenotype caused by overexpression of P-gp (KBv200 cells), were treated with anti-Fas (CH-11 monoclonal antibody) to induce apoptosis. Cytotoxicity was detected by MTT assay. Symptoms of cell death were assessed by morphological observation after Hoechst33258 staining, activation of caspase-8 and caspase-3 was measured by Western blotting. Compared with KB cells, the resistance of KBv200 cells to VCR (vincristine) was about 51-fold higher. Anti-Fas (CH-11) induced cytotoxicity and apoptosis in both sensitive KB cells and MDR phenotype KBv200 cells. The IC50 of CH-11 in KB cells was similar to that in KBv200 cells. CH-11 induced similar apoptotic rates in both KB cells and KBv200 cells, which could be classified as caspase-dependent apoptotic pathway. Verapamil (VRP) did not affect CH-11-mediated apoptosis in KBv200 cells. Expression of P-glycoprotein does not induce resistance to caspase-8 and -3 activation or anti-Fas-induced cell apoptosis.

(PDF emailed within 1 workday: $29.90)

Accession: 049839866

Download citation: RISBibTeXText

PMID: 15962371

Related references

Arsenic trioxide induced cell apoptosis by mitochondria dependent pathway in KB and KBv200 cells. Ai Zheng 23(6): 631-634, 2004

Anthracenedione derivative 1403P-3 induces apoptosis in KB and KBv200 cells via reactive oxygen species-independent mitochondrial pathway and death receptor pathway. Cancer Biology and Therapy 6(9): 1413-1421, 2007

Quercetin induces apoptosis via the mitochondrial pathway in KB and KBv200 cells. Journal of Agricultural and Food Chemistry 61(9): 2188-2195, 2014

Lgf-YL-9 induces apoptosis in human epidermoid carcinoma KB cells and multidrug resistant KBv200 cells via reactive oxygen species-independent mitochondrial pathway. Cancer Letters 249(2): 256-270, 2006

Apoptosis of human oral epidermoid carcinoma KB cells and multidrug resistant KBv200 cells induced by matrine. Hua Xi Kou Qiang Yi Xue Za Zhi 23(3): 254-257, 2005

A ROS-mediated mitochondrial pathway and Nrf2 pathway activation are involved in BDE-47 induced apoptosis in Neuro-2a cells. Chemosphere 184: 679-686, 2017

Apoptosis of human carcinoma of mouth floor KB cells and multidrug resistant KBv200 cells induced by azide methyl anthraquinone derivative. Yao Xue Xue Bao 40(1): 22-26, 2005

PI3-kinase/Akt-Mcl1 signal pathway is involved in IL-6 regulated anti-apoptosis activity to UVB in BCC cells. Journal of Investigative Dermatology 117(2): 486, 2001

Inhibition of P-glycoprotein by wogonin is involved with the potentiation of etoposide-induced apoptosis in cancer cells. Annals of the New York Academy of Sciences 1171: 132-136, 2009

Cadmium -induced apoptosis and anti-apoptosis are linked through production of reactive oxygen species , activation of NF-kappaB and overexpression of multidrug resistance P-glycoprotein in rat kidney proximal tubule cells. Journal of the American Society of Nephrology 10(PROGRAM AND ABSTR ISSUE): 652A, Sept, 1999

NF-kappaB pathway is involved in griseofulvin-induced G2/M arrest and apoptosis in HL-60 cells. Journal of Cellular Biochemistry 101(5): 1165-1175, 2007

Activation of the apoptosis signal-regulating kinase 1/c-Jun N-terminal kinase pathway is involved in the casticin-induced apoptosis of colon cancer cells. Experimental and Therapeutic Medicine 8(5): 1494-1500, 2014

A novel podophyllotoxin derivative (YB-1EPN) induces apoptosis and down-regulates express of P-glycoprotein in multidrug resistance cell line KBV200. European Journal of Pharmacology 627(1-3): 69-74, 2010

The role of Pten/Akt signaling pathway involved in BPA-induced apoptosis of rat Sertoli cells. Environmental Toxicology 30(7): 793-802, 2016

NMDA receptor-mediated ERK 1/2 pathway is involved in PFHxS-induced apoptosis of PC12 cells. Science of the Total Environment 491-492: 227-234, 2014