+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Polysaccharide/polynucleotide complexes. Part 6: complementary-strand-induced release of single-stranded DNA bound in the schizophyllan complex

Polysaccharide/polynucleotide complexes. Part 6: complementary-strand-induced release of single-stranded DNA bound in the schizophyllan complex

Chemistry and Biodiversity 1(3): 520-529

Spectroscopic properties of single-stranded DNA/schizophyllan ternary complexes (ss-DNA2s-SPG), induced by addition of either complementary or noncomplementary strands, have been investigated. The addition of the complementary strands to ss-DNA2s-SPG induced the quick release of the bound ss-DNA to the complementary strands (both DNA and RNA), whereas the ternary complex was unaffected upon addition of noncomplementary strands. Our experiments imply that SPG has complexation properties indispensable to the gene carriers. As far as we know, there is no report on exploitation of such nonviral gene carriers that can accomplish an intelligent release of the bound ss-DNA toward the complementary strands. We believe, therefore, that SPG, a natural and neutral polysaccharide, has a great potential to become a new ss-DNA carrier.

(PDF emailed within 0-6 h: $19.90)

Accession: 049949700

Download citation: RISBibTeXText

PMID: 17191866

DOI: 10.1002/cbdv.200490045

Related references

Polysaccharide--polynucleotide complexes. 2. Complementary polynucleotide mimic behavior of the natural polysaccharide schizophyllan in the macromolecular complex with single-stranded RNA and DNA. Biomacromolecules 2(3): 641-650, 2001

Polysaccharide-polynucleotide complexes. Part 7. Hydrogen-ion and salt concentration dependence of complexation between schizophyllan and single-stranded homo RNAs. Bioorganic Chemistry 31(3): 216-226, 2003

Polysaccharide-polynucleotide complexes (IV): antihydrolysis effect of the schizophyllan/poly(C) complex and the complex dissociation induced by amines. Bioorganic Chemistry 29(3): 178-185, 2001

Polysaccharide-polynucleotide complexes VIII. Cation-induced complex formation between polyuridylic acid and schizophyllan. Biopolymers 65(1): 1-9, 2002

Polysaccharide-polynucleotide complexes Thermal stability of schizophyllan /poly triple strands is controllable by alpha-amino acid modification. Bioorganic Chemistry 31(2): 163-171, 2003

Polysaccharide-polynucleotide complexes. Part 32. Structural analysis of the curdlan/poly(cytidylic acid) complex with semiempirical molecular orbital calculations. Biomacromolecules 6(3): 1540-1546, 2005

Direct sequencing of double-stranded DNA PCR products via removing the complementary strand with single-stranded DNA of an M13 clone. Nucleic Acids Research 18(4): 1076, 1990

One strand of ars189 from the maxicircle of Crithidia fasciculata transforms Saccharomyces cerevisiae more efficiently than its complementary strand as a single stranded DNA. Gene 40(2-3): 285-290, 1985

Primase from Escherichia coli primes single-stranded templates in the absence of single-stranded DNA-binding protein or other auxiliary proteins. Template sequence requirements based on the bacteriophage G4 complementary strand origin and Okazaki fragment initiation sites. Journal of Biological Chemistry 268(17): 12970-6, 1993

Hydroxyl radical-induced strand break formation in single-stranded polynucleotides and single-stranded DNA in aqueous solution as measured by light scattering and by conductivity. International Journal of Radiation Biology 54(5): 723-737, 1988

Equilibrium binding of Escherichia coli single-strand binding protein to single-stranded nucleic acids in the (SSB)65 binding mode. Cation and anion effects and polynucleotide specificity. Biochemistry 27(1): 456-471, 1988

Complementary-addressed modification of double-stranded DNA in a triple-strand complex. Doklady Akademii Nauk SSSR 300(4): 1006-1009, 1988

Initiation signals for complementary strand DNA synthesis on single-stranded plasmid DNA. Nucleic Acids Research 11(14): 4957-4975, 1983

Influence of 2'-O-alkylation on the structure of single-stranded polynucleotides and the stability of 2'-O-alkylated polynucleotide complexes. Biochemistry 13(13): 2762-2771, 1974

Stability and dynamics of the complexes of the single strand DNA binding protein (E.coli) with single stranded dna. European Biophysics Journal 7(4): 280-280, 1981