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Prediction of protein retention in hydrophobic interaction chromatography


Prediction of protein retention in hydrophobic interaction chromatography



Biotechnology Advances 23(5): 359-368



ISSN/ISBN: 0734-9750

PMID: 15894452

DOI: 10.1016/j.biotechadv.2005.04.005

Hydrophobic interaction chromatography (HIC) is a powerful technique for protein separation. This review examines methodologies for predicting protein retention time in HIC involving elution with salt gradients. The methodologies discussed consider three-dimensional structure data of the protein and its surface hydrophobicity. Despite their limitations, the methods discussed are useful in designing purification processes for proteins and easing the tedious experimental work that is currently required for developing purification protocols.

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Accession: 049976338

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