EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Prokaryotic expression of an antigen gene of Trichinella spiralis and identification of the recombinant protein


Prokaryotic expression of an antigen gene of Trichinella spiralis and identification of the recombinant protein



Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 22(5): 257-261



ISSN/ISBN: 1000-7423

PMID: 15830873

To obtain the recombinant protein of an antigen gene Ts88 of Trichinella spiralis and identify the characteristics of the recombinant protein. Ts88 cDNA obtained by immunoscreening the cDNA library of adult T. spiralis was subcloned into the pET-28c(+) expression vector and expressed in E. coli. Mice were immunized with the fusion protein incorporated into Freund' s adjuvant and the immune sera were collected. The titers of the Ts88 immune sera and the antigenicity of the recombinant protein were detected by ELISA and Western blotting. Immuno-fluorescence test was performed in order to confirm the distribution of Ts88 protein in the worm. The fragment of Ts88 gene was expressed successfully in E. coli and a highly purified fusion protein was obtained. Immunization with the recombinant protein in mice produced high titers of antibodies, which recognized some components of native antigens of soluble proteins from adult worm of T. spiralis. Western blotting analysis showed that Ts88 recombinant antigen was recognized by all the positive sera, such as the sera from infected or immunized rabbits, from infected swine and from patients of trichinosis. Immuno-fluorescence test confirmed that Ts88 protein mainly distributed in the cuticle surface of the worm. The Ts88 antigen gene from T. spiralis was successfully expressed. The recombinant protein presented antigenicity.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 050041574

Download citation: RISBibTeXText

Related references

Prokaryotic expression of an antigen gene of Trichinella spiralis and identification of the recombinant protein. Chinese Journal of Parasitology and Parasitic Diseases ober 30; 22(5): 257-261, 2004

Prokaryotic expression of the antigen gene Ts43 of Trichinella spiralis and identification of its recombinant protein. Chinese Journal of Zoonoses 24(6): 539-542, 2008

Prokaryotic expression of Trichinella spiralis gene Ts21 and identification of the recombinant protein. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 25(6): 442-446, 2007

Prokaryotic expression and characterization of an antigenic gene of adult Trichinella spiralis. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 21(1): 16-19, 2003

Detection of anti-Trichinella spiralis antibody by indirect ELISA using recombinant protein as antigen. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 23(3): 143-145, 2005

Protective immunity in mice after immunized with recombinant fusion protein encoding p46 kDa antigen of Trichinella spiralis. Chinese Journal of Zoonoses 21(3): 221-224, 2005

Antigen mediated release of eosinophil growth stimulating factor from trichinella spiralis sensitized spleen cells a comparison of trichinella spiralis stage specific antigen preparations. Immunology 45(4): 605-612, 1982

Cloning and expression of WN1 antigen gene from newborn larvae of Trichinella spiralis. Chinese Journal of Veterinary Science 25(3): 276-278, 2005

Studies on in vitro expression condition of antigen p49 gene from Trichinella spiralis. Chinese Journal of Zoonoses 14(2): 7-10, 1998

Expression conditions in vitro for the antigen gene Ts21 of Trichinella spiralis. Immunological Journal: 6, 697-699, 2008

Identification of transcription and expression of Trichinella spiralis T668 gene. Chinese Journal Of Veterinary Science: 11, 1292-1295, 2008

Prokaryotic expression and activity identification of gene recombinant protein of brain-derivedneurotrophic factor precursor. Zhonghua Yi Xue Za Zhi 94(31): 2460-2463, 2014

Immunoscreening of Trichinella spiralis cDNA clones and prokaryotic expression. Chinese Journal of Parasitic Disease Control 16(1): 7-9, 2003

Expression in COS7 cell line of Ts87 gene encoding antigen from Trichinella spiralis. Chinese Journal of Parasitic Disease Control 16(2): 71-73, 2003

Cloning and expression of the antigen structural gene TspE1 from Pre-encysted larvae of Trichinella spiralis. Chinese Journal of Parasitology and Parasitic Diseases ober 30; 20(5): 278-280, 2002