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Protein heterodimerization through ligand-bridged multivalent pre-organization: enhancing ligand binding toward both protein targets

Protein heterodimerization through ligand-bridged multivalent pre-organization: enhancing ligand binding toward both protein targets

Journal of the American Chemical Society 127(7): 2044-2045

Structure-based design of a bifunctional ligand for two protein pentamers, cholera toxin B pentamer (CTB) and human serum amyloid P component (SAP), leads to multivalent dimerization of CTB and SAP in solution. This multivalent heterodimerization of proteins significantly enhances the affinity of the bifunctional ligand toward both target proteins.

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Accession: 050054544

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PMID: 15713072

DOI: 10.1021/ja043817r

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