+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Proteotypic classification of spontaneous and transgenic mammary neoplasms



Proteotypic classification of spontaneous and transgenic mammary neoplasms



Breast Cancer Research 6(6): R668



Mammary tumors in mice are categorized by using morphologic and architectural criteria. Immunolabeling for terminal differentiation markers was compared among a variety of mouse mammary neoplasms because expression of terminal differentiation markers, and especially of keratins, provides important information on the origin of neoplastic cells and their degree of differentiation. Expression patterns for terminal differentiation markers were used to characterize tumor types and to study tumor progression in transgenic mouse models of mammary neoplasia (mice overexpressing Neu (Erbb2), Hras, Myc, Notch4, SV40-TAg, Tgfa, and Wnt1), in spontaneous mammary carcinomas, and in mammary neoplasms associated with infection by the mouse mammary tumor virus (MMTV). On the basis of the expression of terminal differentiation markers, three types of neoplasm were identified: first, simple carcinomas composed exclusively of cells with a luminal phenotype are characteristic of neoplasms arising in mice transgenic for Neu, Hras, Myc, Notch4, and SV40-TAg; second, 'complex carcinomas' displaying luminal and myoepithelial differentiation are characteristic of type P tumors arising in mice transgenic for Wnt1, neoplasms arising in mice infected by the MMTV, and spontaneous adenosquamous carcinomas; and third, 'carcinomas with epithelial to mesenchymal transition (EMT)' are a characteristic feature of tumor progression in Hras-, Myc-, and SV40-TAg-induced mammary neoplasms and PL/J and SJL/J mouse strains, and display de novo expression of myoepithelial and mesenchymal cell markers. In sharp contrast, EMT was not detected in papillary adenocarcinomas arising in BALB/cJ mice, spontaneous adenoacanthomas, neoplasms associated with MMTV-infection, or in neoplasms arising in mice transgenic for Neu and Wnt1. Immunohistochemical profiles of complex neoplasms are consistent with a stem cell origin, whereas simple carcinomas might originate from a cell committed to the luminal lineage. In addition, these results suggest that the initiating oncogenic events determine the morphologic features associated with cancer progression because EMT is observed only in certain types of neoplasm.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 050056770

Download citation: RISBibTeXText

PMID: 15535849

DOI: 10.1186/bcr930


Related references

Mammary gland neoplasms in the bitch. I. Epidemiological aspects. II. Classification of benign neoplasms. III. Classification of malignant neoplasms. Anales de la Facultad de Veterinaria de Leon 29: 61-98, 1983

Spontaneous mammary adenocarcinomas in transgenic mice that carry and express mammary tumor virus myc fusion genes. Cell 38(3): 627-638, 1984

Prevalence and classification of spontaneous mammary intraepithelial lesions in dogs without clinical mammary disease. Veterinary Pathology 47(2): 275-284, 2010

Classification of feline mammary neoplasms. Atti della Societa Italiana delle Scienze Veterinarie 1986; 39(2): 552-554, 1985

Classification of mammary neoplasms in dogs. Deutsche Tierärztliche Wochenschrift 65: 458-461, 1958

Spontaneous mammary neoplasms of the domestic animals. Proceedings of the Royal Society of Medicine 50(8): 557-560, 1957

Pathology of spontaneous mammary neoplasms of dogs in Bulgaria. Obshcha i Sravnitelna Patologiya 24: 12-16, 1988

Metastasising spontaneous mammary gland neoplasms in line A-Sn mice. Voprosy Onkologii 16(11): 104-106, 1970

Clonogenicity and experimental metastatic potential of spontaneous mouse mammary neoplasms. Journal of the National Cancer Institute 77(2): 529-535, 1986

Perspectives for improved and more accurate classification of canine mammary gland neoplasms. Veterinary Pathology 50(2): 347-348, 2013

Biologic behavior of canine mammary neoplasms based on a histogenetic classification. Veterinary Pathology 11(3): 212-229, 1974

Pathomorphological assessment and classification of spontaneous neoplasms in the dog. Medycyna Weterynaryjna 48(5): 200-201, 1992

Prevention of spontaneous mammary adenocarcinoma in HER-2/neu transgenic mice by foreign DNA. Faseb Journal 16(13): 1749-1754, 2002

Chemoprevention of spontaneous mammary tumorigenesis in p53-deficient Wnt-1 transgenic mice. Proceedings of the American Association for Cancer Research Annual Meeting (41): 83, 2000

Lymph nodes, serum proteins in mice with spontaneous neoplasms. II. Structural changes in lymph nodes during the course of spontaneous mammary cancer. Archives of Pathology 71: 532-537, 1961