+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Quantitative determination of heme for forensic characterization of bacillus spores using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry



Quantitative determination of heme for forensic characterization of bacillus spores using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry



Analytical Chemistry 76(10): 2836-2841



A quantitative method was developed for the determination of heme (ferriprotoporphyrin IX) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The method was designed for forensic characterization of the use of blood agar in preparation of Bacillus spores. An alkali wash of 0.3 M ammonium hydroxide was used to solubilize heme from spore samples. The wash was concentrated and analyzed by MALDI-TOFMS. Experimental parameters were optimized to obtain the best signal intensity, maximize signal reproducibility, and improve day-to-day repeatability of the measurement. Sinapinic acid was found to be the best matrix. A sandwich sample preparation protocol was determined to increase the shot-to-shot and point-to-point reproducibility of the measurement. Cobalt(III) protoporphyrin was used as an internal standard and the analyte/internal standard ratio responses from solutions of known concentrations were used to construct a calibration curve (R(2) = 0.993). Limits of detection and quantitation for heme were calculated to be approximately 0.4 (200 fmol) and 0.8 microM (400 fmol), respectively. Spore samples prepared on blood agar and nonblood agar were analyzed using the method. Heme was detected at a concentration of approximately 0.3 ng/mg of spore on samples prepared on blood agar and purified by extensive washing. Heme was not detected on spore samples prepared without blood.

(PDF emailed within 0-6 h: $19.90)

Accession: 050090238

Download citation: RISBibTeXText

PMID: 15144195

DOI: 10.1021/ac034959r


Related references

Rapid characterization of spores of Bacillus cereus group bacteria by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry. Applied and Environmental Microbiology 66(9): 3828-3834, 2000

Characterization of covalently inhibited extracellular lipase from Streptomyces rimosus by matrix-assisted laser desorption/ionization time-of-flight and matrix-assisted laser desorption/ionization quadrupole ion trap reflectron time-of-flight mass spectrometry: localization of the active site serine. Journal of Mass Spectrometry 39(12): 1474-1483, 2004

Characterization of Aspergillus spores by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry 14(24): 2393-2400, 2000

Chemically-assisted fragmentation combined with multi-dimensional liquid chromatography and matrix-assisted laser desorption/ionization post source decay, matrix-assisted laser desorption/ionization tandem time-of flight or matrix-assisted laser desorption/ionization tandem mass spectrometry for improved sequencing of tryptic peptides. European Journal of Mass Spectrometry 11(2): 169-179, 2005

Quantitative determination of peptides using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Biotechniques Suppl: 13-17, 2006

Quantitative determination of proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Analytical Chemistry 66(9): 1408-1415, 1994

Rapid identification of Bacillus anthracis spores in suspicious powder samples by using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Applied and Environmental Microbiology 79(17): 5372-5383, 2014

Factors contributing to peak broadening and mass accuracy in the characterization of intact spores using matrix-assisted laser desorption/ionization coupled with time-of-flight mass spectrometry. Journal of Mass Spectrometry 36(8): 929-936, 2001

Fast quantitative determination of melamine and its derivatives by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry 25(19): 2844-2850, 2011

Quantitative determination of native and methylated cyclodextrins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Carbohydrate Research 286(0): 41-53, 1996

Importance of matrix:analyte ratio for buffer tolerance using 2,5-dihydroxybenzoic acid as a matrix in matrix-assisted laser desorption/ionization-Fourier transform mass spectrometry and matrix-assisted laser desorption/ionization-time of flight. Journal of the American Society for Mass Spectrometry 9(8): 805-813, 1998

Quantitative applications of matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry Determination of copolymer composition in bacterial copolyesters. Rapid Communications in Mass Spectrometry 7(11): 1033-1036, 1993

Quantitative determination of cyclic-di-GMP concentrations in nucleotide extracts of bacteria by Matrix Assisted Laser Desorption Ionization a“ Time of Flight mass spectrometry. 2009

Evaluation of combined matrix-assisted laser desorption/ionization time-of-flight and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry experiments for peptide mass fingerprinting analysis. Rapid Communications in Mass Spectrometry 25(13): 1881-1892, 2011