EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza a and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4



Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza a and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4



Journal of Clinical Microbiology 42(4): 1564-1569



Laboratory diagnosis of viral respiratory infections is generally performed by virus isolation in cell culture and immunofluorescent assays. Reverse transcriptase PCR is now recognized as a sensitive and specific alternative for detection of respiratory RNA viruses. A rapid real-time multiplex PCR assay was developed for the detection of influenza A and influenza B viruses, human respiratory syncytial virus (RSV), parainfluenza virus 1 (PIV1), PIV2, PIV3, and PIV4 in a two-tube multiplex reaction which used molecular beacons to discriminate the pathogens. A total of 358 respiratory samples taken over a 1-year period were analyzed by the multiplex assay. The incidence of respiratory viruses detected in these samples was 67 of 358 (19%) and 87 of 358 (24%) by culture and real-time PCR, respectively. Culture detected 3 influenza A virus, 2 influenza B virus, 57 RSV, 2 PIV1, and 2 PIV3 infections. All of these culture-positive samples and an additional 5 influenza A virus, 6 RSV, 2 PIV1, 1 PIV2, 1 PIV3, and 3 PIV4 infections were detected by the multiplex real-time PCR. The application of real-time PCR to clinical samples increases the sensitivity for respiratory viral diagnosis. In addition, results can be obtained within 6 h, which increases clinical relevance. Therefore, use of this real-time PCR assay would improve patient management and infection control.

(PDF emailed within 0-6 h: $19.90)

Accession: 050111725

Download citation: RISBibTeXText

PMID: 15071005

DOI: 10.1128/JCM.42.4.1564-1569.2004



Related references

Rapid simultaneous diagnosis of infections with respiratory syncytial viruses A and B, influenza viruses A and B, and human parainfluenza virus types 1, 2, and 3 by multiplex quantitative reverse transcription-polymerase chain reaction-enzyme hybridization assay (Hexaplex). Clinical Infectious Diseases 26(6): 1397-1402, 1998

Clinical manifestations and rapid virological diagnosis through an enzyme immunoassay with respiratory infections due to respiratory syncytial virus adenovirus parainfluenza viruses 1 3 and influenza type a and b viruses. Helvetica Paediatrica Acta 40(2-3): 200, 1985

Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR. Journal of Clinical Microbiology 49(4): 1653-1656, 2011

Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms. Journal of Clinical Microbiology 45(7): 2260-2262, 2007

Evaluation of the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for detection of influenza A and influenza B viruses and respiratory syncytial viruses in children. Journal of Clinical Microbiology 46(2): 789-791, 2007

Development of a rapid automated influenza A, influenza B, and respiratory syncytial virus A/B multiplex real-time RT-PCR assay and its use during the 2009 H1N1 swine-origin influenza virus epidemic in Milwaukee, Wisconsin. Journal of Molecular Diagnostics 12(1): 74-81, 2010

A potential rapid immunoperoxidase method of diagnosis in infections due to the respiratory syncytial virus, parainfluenza viruses and adenoviruses. Bacteriologia, Virusologia, Parazitologia, Epidemiologia 41(3-4): 145-146, 1996

Monitoring epidemic viral respiratory infections using one-step real-time triplex RT-PCR targeting influenza A and B viruses and respiratory syncytial virus. Journal of Medical Virology 83(4): 695-701, 2011

Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses. Journal of Clinical Microbiology 42(1): 45-51, 2004

Contribution of respiratory syncytial virus, influenza and parainfluenza viruses to acute respiratory infections in San Luis Potosí, Mexico. Pediatric Infectious Disease Journal 24(12): 1049-1052, 2005

Performance testing of two new one-step real time PCR assays for detection of human influenza and avian influenza viruses isolated in humans and respiratory syncytial virus. Journal of Preventive Medicine and Hygiene 47(4): 127-133, 2007

Evaluation of a multiplex real-time polymerase chain reaction assay for the detection of influenza and respiratory syncytial viruses. Diagnostic Microbiology and Infectious Disease 84(1): 40-42, 2016

The complement fixing antibodies to respiratory viruses influenza a 1 influenza b influenza c parainfluenza 1 sendai respiratory syncytial adenovirus and echo 11 and mycoplasma pneumoniae in healthy persons. Annali Sclavo 18(4): 521-533, 1976

Comparison of the BD Directigen Flu A+B Kit and the Abbott TestPack RSV with a multiplex RT-PCR ELISA for rapid detection of influenza viruses and respiratory syncytial virus. Clinical Microbiology and Infection 11(10): 848-850, 2005

Evaluation of Allplex Respiratory Panel 1/2/3 Multiplex Real-Time PCR Assays for the Detection of Respiratory Viruses with Influenza A Virus subtyping. Annals of Laboratory Medicine 38(1): 46-50, 2017