+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Rubia cordifolia, Fagonia cretica linn and Tinospora cordifolia exert neuroprotection by modulating the antioxidant system in rat hippocampal slices subjected to oxygen glucose deprivation



Rubia cordifolia, Fagonia cretica linn and Tinospora cordifolia exert neuroprotection by modulating the antioxidant system in rat hippocampal slices subjected to oxygen glucose deprivation



Bmc Complementary and Alternative Medicine 4: 11



The major damaging factor during and after the ischemic/hypoxic insult is the generation of free radicals, which leads to apoptosis, necrosis and ultimately cell death. Rubia cordifolia (RC), Fagonia cretica linn (FC) and Tinospora cordifolia (TC) have been reported to contain a wide variety of antioxidants and have been in use in the eastern system of medicine for various disorders. However, their mechanism of action was largely unknown. We therefore selected these herbs for the present study to test their neuroprotective ability and the associated mechanism in rat hippocampal slices subjected to oxygen-glucose deprivation (OGD). Hippocampal Slices were subjected to OGD (oxygen glucose deprivation) and divided into 3 groups: control, OGD and OGD + drug treated. Cytosolic Cu-Zn superoxide dismutase (Cu-Zn SOD), reduced glutathione (GSH), glutathione peroxidase (GPx), nitric oxide (NO) was measured as nitrite (NO2) in the supernatant and protein assays were performed in the respective groups at various time intervals. EPR was used to establish the antioxidant effect of RC, FC and TC with respect to superoxide anion (O2.-), hydroxyl radicals (. OH), nitric oxide (NO) radical and peroxynitrite anion (ONOO) generated from pyrogallol, menadione, DETA-NO and Sin-1 respectively. RT-PCR was performed for the three groups for GCLC, iNOS, Cu-Zn SOD and GAPDH gene expression. All the three herbs were effective in elevating the GSH levels, expression of the gamma-glutamylcysteine ligase and Cu-Zn SOD genes. The herbs also exhibited strong free radical scavenging properties against reactive oxygen and nitrogen species as studied by electron paramagnetic resonance spectroscopy. In addition all the three herbs significantly diminished the expression of iNOS gene after 48 hours which plays a major role in neuronal injury during hypoxia/ischemia. RC, FC and TC therefore attenuate oxidative stress mediated cell injury during OGD and exert the above effects at both the cytosolic as well as at gene expression level and may be an effective therapeutic tool against ischemic brain damage.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 050242312

Download citation: RISBibTeXText

PMID: 15310392

DOI: 10.1186/1472-6882-4-11


Related references

Effect of Rubia cordifolia, Fagonia cretica linn, and Tinospora cordifolia on free radical generation and lipid peroxidation during oxygen-glucose deprivation in rat hippocampal slices. Biochemical and Biophysical Research Communications 324(2): 588-596, 2004

Revision of the genus rubia d in india and adjoining regions rubia aitchisonii d new species rubia cordifolia var cordifolia f strigosa d new form rubia albicaulis var stenophylla f infundibularis d new combination. Bulletin of the Botanical Survey of India 10(1): 1-16, 1968

Comparative immunomodulation potential of Tinospora cordifolia (Willd.) Miers ex Hook. F., Tinospora sinensis (Lour.) Merrill and Tinospora cordifolia growing on Azadirachta indica A. Juss. Indian Journal of Experimental Biology 52(8): 808-813, 2014

Mechanisms of 1S,3R-ACPD-induced neuroprotection in rat hippocampal slices subjected to oxygen and glucose deprivation. Neuropharmacology 35(8): 1037-1048, 1996

Galantamine and memantine produce different degrees of neuroprotection in rat hippocampal slices subjected to oxygen-glucose deprivation. Neuroscience Letters 365(2): 132-136, 2004

Neuroprotection by nicotine in hippocampal slices subjected to oxygen-glucose deprivation: involvement of the alpha7 nAChR subtype. Journal of Molecular Neuroscience 30(1-2): 61-62, 2006

Neuroprotection Promoted by Guanosine Depends on Glutamine Synthetase and Glutamate Transporters Activity in Hippocampal Slices Subjected to Oxygen/Glucose Deprivation. Neurotoxicity Research 29(4): 460-468, 2016

Comparative studies of rubia cordifolia linn. And rubia tinctorum linn (rubiaceae)(*). Ancient Science of Life 13(1-2): 165-179, 1993

Germacranolides of erlangea cordifolia isolation and structures of cordifolia 54 cordifolia 55 cordifolia p 2 and cordifolia 31 by spectral and x ray methods. Journal of the Chemical Society Perkin Transactions I (4): 819-824, 1984

In vivo evaluation of antioxidant activity of alcoholic extract of Rubia cordifolia Linn. and its influence on ethanol-induced immunosuppression. Indian Journal of Pharmacology 35(4): 232-236, 2003

Induction of apoptosis by methanolic extract of Rubia cordifolia Linn in HEp-2 cell line is mediated by reactive oxygen species. Asian Pacific Journal of Cancer Prevention 13(6): 2753-2758, 2012

Effect of RC-18, a new anticancer principle isolated from Rubia cordifolia, Linn. on cell cycle of P388 tumour system. Biomedicine and PharmacoTherapy 36(2): 104-105, 1982

Two new anthraquinones from the roots of Rubia cordifolia Linn. Die Pharmazie 58(3): 216-217, 2003

A comprehensive review of Rubia cordifolia Linn. Pharmacognosy Reviews: 3, 124-134, 2008

Neuroprotection by JM-20 against oxygen-glucose deprivation in rat hippocampal slices: Involvement of the Akt/GSK-3β pathway. Neurochemistry International 90: 215-223, 2015