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Screening the antibody mimic peptide binding to hepatocellular carcinoma cells by phage display technique



Screening the antibody mimic peptide binding to hepatocellular carcinoma cells by phage display technique



Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 21(4): 452-455



To screen the antibibody mimic peptide binding to hepatocellular carcinoma (HCC) from the circular 7-mer peptide libraries. The enrichment of phage was achieved by repetitious biopanning. The affinity of peptide was evaluated by ELISA, and the binding site of the peptide was analysed by the competition ELISA. The output of phage gradually rose along with increasing of biopanning times. The obtained circular 7-mer peptide showed good binding activity to hepatocellular carcinoma cell lines SMMC-7721 and BEL-7402 (P<0.05), and its affinity to SMMC-7721 cells was superior to that of BEL-7402 (P<0.05). At the level of alpha=0.01, the screened 7-mer peptide monoclonal stocks could obviously compete with scFv to bind SMMC-7721 cells (0.005

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Accession: 050264145

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PMID: 15989789


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