+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Selective down-regulation of angiotensin Ii receptor type 1A signaling by protein tyrosine phosphatase SHP-2 in vascular smooth muscle cells

Selective down-regulation of angiotensin Ii receptor type 1A signaling by protein tyrosine phosphatase SHP-2 in vascular smooth muscle cells

Cellular Signalling 16(3): 301-311

ISSN/ISBN: 0898-6568

PMID: 14687660

DOI: 10.1016/j.cellsig.2003.08.001

The heptahelical AT(1) G-protein-coupled receptor lacks inherent tyrosine kinase activity. Angiotensin II binding to AT(1) nevertheless activates several tyrosine kinases and stimulates both tyrosine phosphorylation and phosphatase activity of the SHP-2 tyrosine phosphatase in vascular smooth muscle cells. Since a balance between tyrosine kinase and tyrosine phosphatase activities is essential in angiotensin II signaling, we investigated the role of SHP-2 in modulating tyrosine kinase signaling pathways by stably transfecting vascular smooth muscle cells with expression vectors encoding wild-type SHP-2 protein or a catalytically inactive SHP-2 mutant. Our data indicate that SHP-2 is an efficient negative regulator of angiotensin II signaling. SHP-2 inhibited c-Src catalytic activity by dephosphorylating a positive regulatory tyrosine 418 within the Src kinase domain. Importantly, SHP-2 expression also abrogated angiotensin II-induced activation of ERK, whereas expression of catalytically inactive SHP-2 caused sustained ERK activation. Thus, SHP-2 likely regulates angiotensin II-induced MAP kinase signaling by inactivating c-Src. These SHP-2 effects were specific for a subset of angiotensin II signaling pathways, since SHP-2 overexpression failed to influence Jak2 tyrosine phosphorylation or Fyn catalytic activity. These data show SHP-2 represents a critical negative regulator of angiotensin II signaling, and further demonstrate a new function for this phosphatase in vascular smooth muscle cells.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 050272677

Download citation: RISBibTeXText

Related references

Regulation of inhibitory protein-kappaB and monocyte chemoattractant protein-1 by angiotensin Ii type 2 receptor-activated Src homology protein tyrosine phosphatase-1 in fetal vascular smooth muscle cells. Molecular Endocrinology 18(3): 666-678, 2004

Angiotensin II stimulates tyrosine phosphorylation and activation of insulin receptor substrate 1 and protein-tyrosine phosphatase 1D in vascular smooth muscle cells. Journal of Biological Chemistry 272(19): 12373-12379, 1997

Redox-sensitive signaling by angiotensin Ii involves oxidative inactivation and blunted phosphorylation of protein tyrosine phosphatase SHP-2 in vascular smooth muscle cells from SHR. Circulation Research 103(2): 149-158, 2008

Angiotensin II induces 3CH134, a protein-tyrosine phosphatase, in vascular smooth muscle cells. Journal of Biological Chemistry 268(35): 26037-26040, 1993

Angiotensin II stimulates expression of a protein tyrosine phosphatase, 3CH134, in rat vascular smooth muscle cells. Circulation 88(4 Part 2): I434, 1993

Protein kinase C dependent regulation of the human angiotensin II receptor type 1 promoter in vascular smooth muscle cells. Circulation 94(8): I227, 1996

Protein tyrosine phosphatase PTPepsilonM negatively regulates PDGF beta-receptor signaling induced by high glucose and PDGF in vascular smooth muscle cells. American Journal of Physiology. Cell Physiology 299(5): C1144-C1152, 2010

Angiotensin II type 1 receptor regulates vascular smooth muscle cell contraction and hypertrophy via tyrosine kinase-dependent signaling pathways. Hypertension 28(3): 532, 1996

Discovery of redox-sensitive protein tyrosine kinase in angiotensin II signaling in vascular smooth muscle cells. FASEB Journal 14(4): A591, 2000

Molecular mechanism of angiotensin II-induced insulin resistance in aortic vascular smooth muscle cells: roles of Protein Tyrosine Phosphatase-1B. Vascular Pharmacology 53(3-4): 160-168, 2010

Expression and regulation of protein tyrosine phosphatase LAR in vascular smooth muscle cells Evidence for a role in proliferation. Circulation 98(17): I327, 1998

Angiotensin II elicits mitogenic activity via insulin receptor substrate-1 and protein tyrosine phosphatase-1D in rat aortic smooth muscle cells. European Heart Journal 17(ABSTR Suppl ): 469, 1996

Cyclic AMP-mediated inhibition of angiotensin II-induced protein synthesis is associated with suppression of tyrosine phosphorylation signaling in vascular smooth muscle cells. Journal of Biological Chemistry 272(43): 26879-26886, 1997

FXR-mediated regulation of angiotensin type 2 receptor expression in vascular smooth muscle cells. Cardiovascular Research 77(3): 560-569, 2008

Caveolin-1 is essential for activation of Rac1 and NAD(P)H oxidase after angiotensin II type 1 receptor stimulation in vascular smooth muscle cells: role in redox signaling and vascular hypertrophy. Arteriosclerosis Thrombosis and Vascular Biology 25(9): 1824-1830, 2005