Subtracted restriction fingerprinting--a new typing technique using magnetic capture of tagged restriction fragments

Terletski, V.; Michael, G.B.; Schwarz, S.

Fems Immunology and Medical Microbiology 41(1): 1-8


ISSN/ISBN: 0928-8244
PMID: 15094162
DOI: 10.1016/j.femsim.2004.01.009
Accession: 050441893

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Molecular typing of bacterial pathogens is an important issue in the epidemiological analysis of emerging infections in humans and animals. Numerous methods have been developed for and applied to a wide variety of bacteria of medical, veterinary and zoonotic importance. The present minireview provides a description of a new typing approach designated subtracted restriction fingerprinting (SRF), its use for typing of Salmonella isolates and a comparison with the most widely used typing techniques for these bacteria. SRF is based on double restriction endonuclease digestion of whole cell DNA, followed by a fill-in reaction with specifically tagged nucleotides and subtractive capture of selected restriction fragments. This results in a reduced number of fragments optimal for separation in standard agarose gels.