Section 51
Chapter 50,474

Susceptibility testing of Mycobacterium tuberculosis: comparison of the BACTEC TB-460 method and flow cytometric assay with the proportion method

DeCoster, D.J.; Vena, R.M.; Callister, S.M.; Schell, R.F.

Clinical Microbiology and Infection the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases 11(5): 372-378


ISSN/ISBN: 1198-743X
PMID: 15819863
DOI: 10.1111/j.1469-0691.2005.01127.x
Accession: 050473328

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Tuberculosis is a leading cause of morbidity and mortality worldwide. Susceptibility testing of the causative agent, Mycobacterium tuberculosis, is critical for control of the disease. This study compared the flow cytometric susceptibility assay with the proportion method and the BACTEC TB-460 system. There was agreement between the flow cytometric and proportion methods for 73 (94%) of 78 isoniazid tests, and complete agreement for 26 ethambutol and rifampicin tests. In contrast, the proportion and BACTEC methods failed to agree for 22%, 15% and 8% of isoniazid, ethambutol and rifampicin tests, respectively. These findings indicated that susceptibility testing by the flow cytometric assay is accurate, with results available within 24 h of initiation of the testing procedure.

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