+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Tetrazolium violet induces G0/G1 arrest and apoptosis in brain tumor cells



Tetrazolium violet induces G0/G1 arrest and apoptosis in brain tumor cells



Journal of Neuro-Oncology 77(2): 109-115



Tetrazolium violet (TV), a potent anticancer agent, has been shown to induce cell growth-inhibition in tumor cells. However, the related mechanism has not been revealed yet. In this report we assessed the influence of TV on cell growth and cell cycle in brain tumor cells. Treatment of C6 tumor cells with TV (5-15 microM for 24-72 h) resulted in a growth inhibition in a dose and time-dependent manner and G0/G1 phase arrest, determined by flow cytometry analysis. These effects were accompanied by apoptosis other than necrosis, evidenced by nuclear condensation, terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay and trypan blue exclusion assay plus lactate dehydrogenase (LDH) release assay. Treatment of cells with TV at 15 microM for 24 h resulted in an increase in the activity of caspase-3, evidenced by colorimetric assay, and a dramatic up-regulation of p53, accompanied with a significant increase of Bax/Bcl-2 ratio, as evidenced by immunofluorescence assay. These results suggest that TV induces growth inhibition of C6 cells through p53-midiated apoptotic pathway and G0/G1 checkpoint mechanism. Although detailed mechanisms remain to be explored, selective blockage of tumor cells in G0/G1 phase accompanied by p53-associated apoptosis makes tetrazolium violet a promising anticancer agent, meriting further investigations.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 050518066

Download citation: RISBibTeXText

PMID: 16314959

DOI: 10.1007/s11060-005-9012-1


Related references

Tetrazolium violet induced apoptosis and cell cycle arrest in human lung cancer a549 cells. Biomolecules and Therapeutics 20(2): 177-182, 2012

Tetrazolium violet induces apoptosis via caspases-8, -9 activation and Fas/FasL up-regulation in rat C6 glioma cells. Archives of Pharmacal Research 32(4): 575-581, 2009

Luteolin induces apoptosis, G0/G1 cell cycle growth arrest and mitochondrial membrane potential loss in neuroblastoma brain tumor cells. Drug Research 65(2): 91-95, 2016

Tetrazolium violet inhibits cell growth and induces cell death in C127 mouse breast tumor cells. Chemico-Biological Interactions 174(1): 19-26, 2008

The p75(NTR) tumor suppressor induces cell cycle arrest facilitating caspase mediated apoptosis in prostate tumor cells. Biochemical and Biophysical Research Communications 341(4): 1184-1192, 2006

Galectin-8 induces growth arrest and apoptosis of tumor cells. Cell Biology International 25(2): A14-A15, 2001

Gli1 induces G2/M arrest and apoptosis in hippocampal but not tumor-derived neural stem cells. Stem Cells 26(4): 1027-1036, 2008

β-ionone induces cell cycle arrest and apoptosis in human prostate tumor cells. Nutrition and Cancer 65(4): 600-610, 2013

The Wilms' tumor suppressor gene WT1 induces G1 arrest and apoptosis in myeloblastic leukemia M1 cells. FEBS Letters 409(1-2): 41-45, 1997

NSC606985, a novel camptothecin analog, induces apoptosis and growth arrest in prostate tumor cells. Cancer ChemoTherapy and Pharmacology 63(2): 303-312, 2009

Histone deacetylase inhibition induces cell cycle arrest and apoptosis in human tumor cells. Proceedings of the American Association for Cancer Research Annual Meeting 39: 450, 1998

T11TS/S-LFA3 induces apoptosis of the brain tumor cells: a new approach to characterise the apoptosis associated genetic changes by arbitrarily primed-PCR. Cancer Letters 222(1): 23-38, 2005

Adenovirus mediated PTEN expression in colorectal tumor cells induces G2/M cell cycle arrest and apoptosis. Proceedings of the American Association for Cancer Research Annual Meeting 43: 1100, 2002

Sunitinib induces apoptosis and growth arrest of medulloblastoma tumor cells by inhibiting STAT3 and AKT signaling pathways. Molecular Cancer Research 8(1): 35-45, 2010

Deoxypodophyllotoxin induces G2/M cell cycle arrest and apoptosis in SGC-7901 cells and inhibits tumor growth in vivo. Molecules 20(1): 1661-1675, 2015