+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The TP53 dependence of radiation-induced chromosome instability in human lymphoblastoid cells

, : The TP53 dependence of radiation-induced chromosome instability in human lymphoblastoid cells. Radiation Research 159(6): 730-736

The dose and TP53 dependence for the induction of chromosome instability were examined in cells of three human lymphoblastoid cell lines derived from WIL2 cells: TK6, a TP53-normal cell line, NH32, a TP53-knockout created from TK6, and WTK1, a WIL2-derived cell line that spontaneously developed a TP53 mutation. Cells of each cell line were exposed to (137)Cs gamma rays, and then surviving clones were isolated and expanded in culture for approximately 35 generations before the frequency and characteristics of the instability were analyzed. The presence of dicentric chromosomes, formed by end-to-end fusions, served as a marker of chromosomal instability. Unexposed TK6 cells had low levels of chromosomal instability (0.002 +/- 0.001 dicentrics/cell). Exposure of TK6 cells to doses as low as 5 cGy gamma rays increased chromosome instability levels nearly 10-fold to 0.019 +/- 0.008 dicentrics/cell. There was no further increase in instability levels beyond 5 cGy. In contrast to TK6 cells, unexposed cultures of WTK1 and NH32 cells had much higher levels of chromosome instability of 0.034 +/- 0.007 and 0.041 +/- 0.009, respectively, but showed little if any effect of radiation on levels of chromosome instability. The results suggest that radiation exposure alters the normal TP53-dependent cell cycle checkpoint controls that recognize alterations in telomere structure and activate apoptosis.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 050559822

PMID: 12751955

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Stauber C.; Altschmied J.; Akerblom I.E.; Marron J.L.; Mellon P.L., 1992: Mutual cross interference between glucocorticoid receptor and creb inhibits transactivation in placental cells. Transcription of the glycoprotein hormone .alpha.-subunit gene in placental cells is repressed by glucocorticoids, an effect that is mediated through the glucocorticoid receptor (GR). Although the DNA-binding domain of GR has been shown to be impo...

Wehner, S.; Behrendt, F.F.; Lyutenski, B.N.; Lysson, M.; Bauer, A.J.; Hirner, A.; Kalff, Jörg.C., 2006: Inhibition of macrophage function prevents intestinal inflammation and postoperative ileus in rodents. Background: Abdominal surgery results in a molecular and cellular inflammatory response in the intestine, leading to postoperative ileus. It was hypothesised that resident macrophages within the intestinal muscularis have an important role in thi...

Jensen D.M.; Machicado G.; Tapia J.; Mautner W.; Franco P., 1980: Endoscopic treatment of telangi ectasia with argon laser photo coagulation and bi polar electro coagulation in patients with chronic gastro intestinal bleeding. Gastrointestinal Endoscopy 26(2): 69

BHARGAVA, K.P.; CHANDRA, O.; VERMA, D.R., 1963: The Mechanism Of The Emetic Action Of Sodium Salicylate. In the dog, the emetic ED50 for sodium salicylate was 256[plus or minus]113 mg/kg for the intravenous route with a mean latency of 10.4[plus or minus]3.96 minutes, and for the oral route 228[plus or minus]68 mg/kg with a mean latency of 18.2[plus...

Hou, H.; Wei, Y.; Song, Y.; Mi, L.; Tang, M.; Li, L.; Fan, Y., 2005: Metal ions play different roles in the third-order nonlinear optical properties of d10 metal-organic clusters. Angewandte Chemie 44(37): 6067-6074

Colder, C.R.; Scalco, M.; Trucco, E.M.; Read, J.P.; Lengua, L.J.; Wieczorek, W.F.; Hawk, L.W., 2014: Prospective associations of internalizing and externalizing problems and their co-occurrence with early adolescent substance use. The literature is equivocal regarding the role of internalizing problems in the etiology of adolescent substance use. In this study, we examined the association of internalizing and externalizing behavior problems and their co-occurrence with earl...

Arispe, Rosario; Rumiz, Damian; Noss, Andrew J., 2007: Six species of cats registered by camera trap surveys of tropical dry forest in Bolivia. The records of six species of wild cats (Leopardus pardalis, L. wiedii, Oncifelis geoffroyi, Herpailurus yaguarondi, Puma concolor and Panthera onca) coexisting in relatively small areas of forest is unique for the Neotropics. Within the economic...

Somjen, D.; Knoll, E.; Vaya, J.; Stern, N.; Tamir, S., 2004: Estrogen-like activity of licorice root constituents: glabridin and glabrene, in vascular tissues in vitro and in vivo. Post-menopausal women have higher incidence of heart diseases compared to pre-menopausal women, suggesting a protective role for estrogen. The recently Women's Health Initiative (WHI) randomized controlled trial concluded that the overall hea...

Wikelski, M. C., 2003: Morphology, performance and fitness in Galapagos marine iguanas. SICB Annual Meeting & Exhibition Final Program & Abstracts : 339

Thomas, H., 1970: Clinical report on the treatment of postoperative pain with a spasmo-analgesic. Medizinische Monatsschrift 24(1): 34-35