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Time-course changes in VEGF expression and capillarity in the early stage of exercise training with Co treatment in rat skeletal muscles



Time-course changes in VEGF expression and capillarity in the early stage of exercise training with Co treatment in rat skeletal muscles



Acta Physiologica Scandinavica 181(2): 225-232



Aim: Cobalt administration was reported to mimic hypoxia. This study was designed to examine the time-course changes in vascular endothelial growth factor (VEGF) expression and capillary geometry in skeletal muscles during endurance training with CoCl2 administration in female Wistar rats. Methods: Exercise training by running lasted for up to 10 days at 25 m min-1 on a 20% gradient, 15-42 min day-1. Rats in the Co2+-treated groups drank water containing 0.01% CoCl2. Serial frozen sections were stained for alkaline phosphatase and dipeptidylpeptidase IV to identify capillary profiles and VEGF-A protein. Results: In the soleus muscle, the density of VEGF-positive capillaries (VEGF-cap) was significantly increased after 6 and 10 days of the Co2+ administration (by 27 and 65% respectively) while the capillary-to-fibre ratio (C : F) first increased after 10 days. The training with Co2+ significantly increased VEGF-cap by 69, 44 and 60%, respectively, after 3, 6 and 10 days. The VEGF-cap was significantly increased after 6 and 10 days of training alone by 38 and 58%, respectively. In a similar extent, both training groups with and without Co2+ showed a significant increase in the C : F ratio after 6 and 10 days. Conclusions: The present results suggest that activation of the cellular oxygen-sensing mechanism induced by Co2+ administration slightly facilitates an expression of VEGF but does not facilitate exercise-induced microvascular remodelling in hind-leg muscles.

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Accession: 050794389

Download citation: RISBibTeXText

PMID: 15180795

DOI: 10.1111/j.1365-201x.2004.01279.x


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