+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

VEGF is up-regulated by hypoxic stimulation and related to tumour angiogenesis and severity of disease in oral squamous cell carcinoma: in vitro and in vivo studies



VEGF is up-regulated by hypoxic stimulation and related to tumour angiogenesis and severity of disease in oral squamous cell carcinoma: in vitro and in vivo studies



International Journal of Oral and Maxillofacial Surgery 35(6): 533-538



The present study was aimed to speculate whether the up-regulation of VEGF in oral squamous cell carcinoma (OSCC) is associated with oxygen levels, tumor angiogenesis and severity of disease. Under different oxygen levels, VEGF protein production in two oral cancer cell lines was quantitatively documented by using ELISA kit. Correlations between expression of VEGF, microvessel density, and various clinico-pathologic factors were studied in forty patients with OSCC. VEGF production was continuously elevated in supernatants from both cell lines in respond to the drop of oxygen levels. When oxygen level decreased to 1%, there was a 2.1-fold and nearly a 2.9-fold elevation of VEGF production in TSCCa and GNM cell line, respectively. On hypoxia VEGF production also presented a time-dependent up-regulation in both oral cancer cell lines. VEGF positivity was correlated with regional lymph nodal involvement and clinical stage. Microvessel density was significantly higher in VEGF-positive tumors than in VEGF-negative tumors. The presence of hypoxia in oral cancers is partly responsible for the up-regulation of VEGF. The elevation of VEGF expression in OSCC tissues correlates with the increased microvessel density and severity of the disease.

(PDF emailed within 0-6 h: $19.90)

Accession: 050922667

Download citation: RISBibTeXText

PMID: 16388929

DOI: 10.1016/j.ijom.2005.09.006


Related references

Angiogenesis-related prognosis in patients with oral squamous cell carcinoma-role of the VEGF +936 C/T polymorphism. Journal of Oral Pathology and Medicine 44(6): 429-436, 2016

Expression and hypoxic regulation of VEGF and angiopoietins in human tumor induced-angiogenesis In vivo and in vitro studies. Society for Neuroscience Abstracts 27(1): 575, 2001

RNA interference-mediated downregulation of hypoxia-inducible factor-1α inhibits angiogenesis and survival of oral squamous cell carcinoma in vitro and in vivo. European Journal of Cancer Prevention 21(3): 289-299, 2012

VEGF expression and angiogenesis in oral squamous cell carcinoma: an immunohistochemical and morphometric study. Clinical and Experimental Medicine 10(4): 209-214, 2011

Immunohistochemical study of tumour angiogenesis in oral squamous cell carcinoma. Oral Oncology 33(5): 369-374, 1998

WISP-1 a novel angiogenic regulator of the CCN family promotes oral squamous cell carcinoma angiogenesis through VEGF-A expression. Oncotarget 6(6): 4239-4252, 2016

Evaluation of expression of midkine in oral squamous cell carcinoma and its correlation with tumour angiogenesis. International Journal of Oral and Maxillofacial Surgery 36(2): 159-164, 2006

Prognostic significance of VEGF-C in predicting micrometastasis and isolated tumour cells in N0 oral squamous cell carcinoma. Journal of Oral Pathology and Medicine 46(3): 194-200, 2016

Expression of pro-inflammatory protein, iNOS, VEGF and COX-2 in oral squamous cell carcinoma (OSCC), relationship with angiogenesis and their clinico-pathological correlation. Medicina Oral, Patologia Oral Y Cirugia Bucal 14(7): E319-E324, 2009

Short interfering RNA targeting Net1 reduces the angiogenesis and tumor growth of in vivo cervical squamous cell carcinoma through VEGF down-regulation. Human Pathology 65: 113-122, 2017

Studies of the serum HER-2/neu and squamous cell carcinoma-related antigen expression in patients with oral squamous cell carcinoma. Journal of Oral Pathology and Medicine 36(2): 83-87, 2007

CD24+ tumor-initiating cells from oral squamous cell carcinoma induce initial angiogenesis in vivo. Microvascular Research 112: 101-108, 2017

Anti-cancer effect of genistein in oral squamous cell carcinoma with respect to angiogenesis and in vitro invasion. Cancer Science 94(2): 215-220, 2003

Abrogation of VEGF expression in human head and neck squamous cell carcinoma decreases angiogenic activity in vitro and in vivo. International Journal of Oncology 23(3): 577-583, 2003

Association of Macrophages With Angiogenesis in Oral Epithelial Dysplasia, Oral Verrucous Carcinoma, and Oral Squamous Cell Carcinoma: An Immunohistochemical Study. Applied Immunohistochemistry and Molecular Morphology 25(3): 203-208, 2015