Whole CagA gene amplification of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism
Ye, S.; Ao, J.; Peng, Y.; Yue, H.; Liao, F.; Hu, G.; Xu, Y.; Zhang, Z.
Journal of Huazhong University of Science and Technology. Medical Sciences 22(4): 276-278
ISSN/ISBN: 1672-0733 PMID: 12674756 DOI: 10.1007/bf02896762
To set up a method of amplification for the whole CagA gene of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism (RFLP), nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind III were used to generate the RFLP fingerprinting. Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained. It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile.