EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

3D modeling and molecular dynamics simulation of an immune-regulatory cytokine, interleukin-10, from the Indian major carp, Catla catla


3D modeling and molecular dynamics simulation of an immune-regulatory cytokine, interleukin-10, from the Indian major carp, Catla catla



Journal of Molecular Modeling 18(5): 1713-1722



ISSN/ISBN: 1610-2940

PMID: 21830101

DOI: 10.1007/s00894-011-1194-1

Interleukin-10 (IL-10) is a pleiotropic immune-regulatory cytokine that is expressed in various species of fish and higher vertebrates, and is activated during infection. In spite of its important role, IL-10 has not been well characterized either functionally or structurally in fish. To analyze its properties and function, we constructed a 3D model of IL-10 in the Indian major carp, the catla (Catla catla), which is a highly preferred fish species and the most commercially important one in the Indian subcontinent. The catla IL-10 model was constructed by comparative modeling using human IL-10 (2ILK) as the template, and a 5 ns molecular dynamics (MD) simulation was carried out to characterize its structural and dynamical features, which was validated by the SAVES, WHAT IF and MolProbity servers. Analysis using the VAST server revealed a comparatively low level of homology between catla and human IL-10 amino acids at the N-terminal (22.7%) compared to the C-terminal (38.29%). Six conserved domains (A-F) were predicted in catla that threaded well with human IL-10, but their putative interaction sites varied significantly. The amino acid residues in helices A and F differed in length between catla and human IL-10, which may lead to the differences in the IL-10/IL-10R complexes of these two species. The existence of two highly conserved amino acid residues (Cys5 and Cys10) in fish IL-10 but not in higher vertebrate (including human) IL-10 was analyzed in this 3D model. CastP, cons-PPISP and InterProSurf server identified several binding pockets with various probe radii, but Cys5 and Cys10 did not form any significant bonds relating to structural stabilization or protein-protein interactions.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 051043403

Download citation: RISBibTeXText

Related references

Molecular characterization, inductive expression and mechanism of interleukin-1 gene induction in the Indian major carp, catla Catla catla. 2012

Dynamic changes in immune-effector activities of Indian major carp, catla Catla catla infected with Edwardsiella tarda. Aquaculture 366 (Nov 5): 62-66, 2012

Morphometric and molecular characterization of Thelohanellus catlae infecting the gills of Indian major carp catla, Catla catla (Hamilton, 1822) from West Bengal, India. Indian Journal of Animal Research 54(7): 883-888, 2020

Influence of dietary phosphorus on mineralization and morphology of Indian major carp, catla (Catla catla) fingerlings. Aquaculture International 19(4): 749-763, 2011

Expression of immune genes in Indian major carp, Catla catla challenged with Flavobacterium columnare. Fish and Shellfish Immunology 94: 599-606, 2019

In vitro effects of bovine lactoferrin, mushroom glucan and Abrus agglutinin on Indian major carp, catla (Catla catla) head kidney leukocytes. Aquaculture 253(1/4): 130-139, 2006

Impact of UV-B radiation on the digestive enzymes and immune system of larvae of Indian major carp Catla catla. International Journal of Radiation Biology 86(3): 181-186, 2010

Genetic variation of wild and hatchery populations of the catla Indian major carp (Catla catla Hamilton 1822: Cypriniformes, Cyprinidae) revealed by RAPD markers. Genetics and Molecular Biology 32(1): 197-201, 2009

Modulation of TLR2, TLR4, TLR5, NOD1 and NOD2 receptor gene expressions and their downstream signaling molecules following thermal stress in the Indian major carp catla (Catla catla). 3 Biotech 5(6): 1021-1030, 2015

A hepatic antimicrobial peptide, hepcidin from Indian major carp, Catla catla: molecular identification and functional characterization. Journal Genetic Engineering and Biotechnology 20(1): 49, 2022

Differential expression profiling of toll like receptors 3, 4 and 9 genes in major tissues of Indian major carp Catla catla. Indian Journal of Animal Sciences 83(9): 983-986, 2013

Biochemical response of the Indian major carp, Catla catla to chromium toxicity. Indian Journal of Environmental Health 37(3): 192-196, 1995

Acute toxicity of synthetic pyrethroids to Indian major carp, Catla catla L. Bulletin of Environmental Contamination and Toxicology 74(3): 610-613, 2005

Implication of melatonin in oocyte maturation in Indian major carp Catla catla. Fish Physiology and Biochemistry 31(2-3): 201-207, 2005

Observations on the condition factor k in an indian major carp catla catla from 2 reservoirs/. Environment India 5(1-2): 1-4, 1982, 1983