+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A 3D aligning method for stimulated emission depletion microscopy using fluorescence lifetime distribution

A 3D aligning method for stimulated emission depletion microscopy using fluorescence lifetime distribution

Microscopy Research and Technique 77(11): 935-940

Optimal resolution by stimulated emission depletion (STED) microscopy requires precise alignment of the donut-shaped depletion focus to the excitation focus. In this article, we demonstrate that fluorescence lifetime distribution can be implemented to align the STED system. Different from the traditional aligning methods in which a scattering imaging module is often equipped, the lifetime-based method is free from probable mismatches between the scattering mode and the fluorescent mode, drift errors caused by separate imaging and complex fitting methods. Based on this method, a spatial resolution of 38 nm by time-gated detection has been achieved.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 051056487

Download citation: RISBibTeXText

PMID: 25111314

DOI: 10.1002/jemt.22420

Related references

Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging. Optics Letters 33(2): 113-115, 2008

Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Optics Letters 19(11): 780-782, 1994

Dual-mode super-resolution imaging with stimulated emission depletion microscopy and fluorescence emission difference microscopy. Applied Optics 54(17): 5425-5431, 2015

Supercontinuum stimulated emission depletion fluorescence lifetime imaging. Journal of Physical Chemistry. B 116(27): 7821-7826, 2012

Increasing fluorescence lifetime for resolution improvement in stimulated emission depletion nanoscopy. Journal of Biophotonics 2018: E201800315-E201800315, 2018

Effects of coherence and vector properties of the light on the resolution limit in stimulated emission depletion fluorescence microscopy. Journal of the Optical Society of America. A, Optics, Image Science, and Vision 25(6): 1378-1382, 2008

Breaking Abbe's diffraction resolution limit in fluorescence microscopy with stimulated emission depletion beams of various shapes. Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics 64(6 Pt 2): 066613-066613, 2001

Auto-aligning stimulated emission depletion microscope using adaptive optics. Optics Letters 38(11): 1860-1862, 2014

Fluorescence lifetime imaging microscopy: two-dimensional distribution measurement of fluorescence lifetime. Methods in Enzymology 414: 633-642, 2006

Stimulated-emission-depletion microscopy with a multicolor stimulated-Raman-scattering light source. Optics Letters 33(21): 2491-2493, 2008

The number of accumulated photons and the quality of stimulated emission depletion lifetime images. Photochemistry and Photobiology 90(4): 767-772, 2015

Fluorescence lifetime imaging microscopy using rapid lifetime determination method Theory and applications. Biophysical Journal 76(1 PART 2): A10, 1999

Multiphoton microscopy and fluorescence lifetime imaging provide a novel method in studying drug distribution and metabolism in the rat liver in vivo. Journal of Biomedical Optics 16(8): 086013-086013, 2012

Long-distance fluorescence lifetime imaging using stimulated emission. Journal of Biomedical Optics 17(1): 011009-011009, 2012

Stimulated Emission Depletion Microscopy. Chemical Reviews 117(11): 7377-7427, 2017