+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A New Flavonoid C-Glycoside from Celtis australis L. and Celtis occidentalis L. Leaves and Potential Antioxidant and Cytotoxic Activities



A New Flavonoid C-Glycoside from Celtis australis L. and Celtis occidentalis L. Leaves and Potential Antioxidant and Cytotoxic Activities



Scientia Pharmaceutica 79(4): 963-975



A major development over the past two decades has been the realization that free radical induced lipid peroxidation and DNA damage are associated with major health problems, e.g. cancer and ageing. Plant-derived antioxidants are increasingly found beneficial in protecting against these diseases. Celtis australis L. and Celtis occidentalis L. are two plants that have a variety of uses in folk medicine but have not been evaluated before for their antioxidant and cytotoxic properties. Therefore, the extracts of both plants' leaves were investigated for these activities, as well as isolation of the bioactive compounds responsible for the activities. Molecular structures of the compounds were elucidated by UV, HRESIMS, 1D ((1)H and (13)C) and 2D ((1)H-(13)C HSQC and (1)H-(13)C HMBC) NMR analyses. The ethanolic and aqueous extracts, n-butanol fractions and the isolated major compound were tested for their antioxidant activity using DPPH radical scavenging assay, xanthine oxidase-induced generation of superoxide radical and lipid peroxidation assay by thiobarbituric acid-reactive substances (TBARS) method using rat tissue homogenates. Cytotoxic activities were studied using standard MTT assay. A novel flavonoid C-triglycoside, 4‴-α-rhamnopyranosyl-2″-O-β-d-galactopyranosylvitexin, was isolated from both plants' leaves, together with seven known flavonoids. The n-butanol fractions and the major compound 2″-O-β-galactopyranosylvitexin showed significant antioxidant activities, more pronounced than the tested standards BHT and dl-α-tocopherol in most tests. All extracts showed variable cytotoxic activities. This study provides strong evidence for the antioxidant and cytotoxic activities of the extracts of Celtis australis L. and Celtis occidentalis L. leaves, which were attributed to the polar n-butanol fractions and the major isolated flavonoid 2″-galactosylvitexin.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 051070000

Download citation: RISBibTeXText

PMID: 22145118

DOI: 10.3797/scipharm.1108-19


Related references

The nettle tree Celtis australis, an exclusive host for the butterfly Libythea celtis in Provence Un hote exclusif du micocoulier de Provence Celtis australis le papillon Libythea celtis. Bulletin de la Societe d'Etude des Sciences Naturelles de Nimes et du Gard. 1989- ; 59: 66-70, 1992

Botanical and genetic characteristics of Celtis australis L and Celtis occidentalis L grown in Egypt. Bulletin of Faculty of Pharmacy Cairo University 49(1): 0-57, 2011

Un hote exclusif du micocoulier de Provence (Celtis australis) le papillon Libythea celtis. 1992

Torus structure and development in the woods of ulmus alata michx. celtis laevigata willd. and celtis occidentalis l. IAWA (International Association of Wood Anatomists) Bulletin 11(1): 71-83, 1990

Analysis of rare flavonoid C-glycosides in Celtis australis L. by micellar electrokinetic chromatography. Journal of Pharmaceutical and Biomedical Analysis 51(5): 1165-1168, 2010

Nutrient dynamics in Celtis australis leaves. Advances in Horticulture and Forestry 2: 194-199, 1992

Seasonal variation in phenolics in leaves of Celtis australis (Cannabaceae). Biochemical Systematics and Ecology 41: 110-114, 2012

Gall-formation on leaves of Celtis occidentalis L. resulting from material injected by Pachypsylla sp. Transactions of the American Microscopical Society, Columbus. 83: 1, 62-78, 1964

Chemical composition and nutritive value of the leaves of khirk tree (Celtis australis Linn. J. Res. Punjab agric. Univ, 6: 4, 942-7. Bibl. 13, 1969

Physiological Effects of Four Gall Types on Leaves of Hackberry ( Celtis occidentalis ) in Northeastern Kansas, U.S.A. Journal of the Kansas Entomological Society 90(4): 304-312, 2017

Phytotoxic Potential of Celtis Australis L. (Family Ulmaceae) Against FOUR CROP SPECIES. Pakistan Journal of Botany 46(6): 2063-2067, 2014

Foliar morphology of Celtis tala and Celtis pallida, with special reference to the domatia. Kurtziana 63-80, 1976

Leaf morphology in celtis tala and celtis pallida with special reference to the domatia. Kurtziana 9: 63-80, 1976

Owoce Celtis z polskiego miocenu; the endocarps of Celtis from the Miocene in Poland. Societas Botanicorum Poloniae, Acta 15(1): 47-51, 1938

Pliocen es pleisztocen Celtis-termesek Magyarorszagbol es Dalmaciabol (Pliozaene und Pleistozaene Celtis-Fruechte aus Ungarn und Dalmatien). Foldtani Kozlony: 153-157, 1933