+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A case of wound dual infection with Pasteurella dagmatis and Pasteurella canis resulting from a dog bite -- limitations of Vitek-2 system in exact identification of Pasteurella species

A case of wound dual infection with Pasteurella dagmatis and Pasteurella canis resulting from a dog bite -- limitations of Vitek-2 system in exact identification of Pasteurella species

European Journal of Medical Research 16(12): 531-536

Pasteurella species, widely known as indigenous organisms in the oral and gastrointestinal floras of many wild and domestic animals, are important pathogens in both animals and humans. Human infections due to Pasteurella species are in most cases associated with infected injuries following animal bites. We encountered a rare case of dual infections caused by different two Pasteurella species occurred in a previously healthy 25-year-old female sustaining injury by a dog-bite. Exudates from the open wound of her dog-bite site, together with the saliva of the dog were submitted for bacteriological examination. Predominantly appearing grayish-white smooth colonies with almost the same colonial properties but slightly different glistening grown on chocolate and sheep blood agar plates were characterized morphologically by Gram's stain, biochemically by automated instrument using Vitek 2 system using GN cards together with commercially available kit system, ID-Test HN-20 rapid panels, and genetically by sequencing the 16S rRNA genes of the organism using a Taq DyeDeoxy Terminator Cycle Sequencing and a model 3100 DNA sequencer instrument. The causative isolates from the dog-bite site were finally identified as P. canis and P. dagmatis from the findings of the morphological, cultural, and biochemical properties together with the comparative sequences of the 16S rRNA genes. Both the isolates were highly susceptible to many antibiotics and the patient was successfully treated with the administration of so-called the first generation cephalosporin, cefazolin followed by so-called the third generation cephalosporin, cefcapene pivoxil. The isolate from the dog was subsequently identified as P. canis, the same species as the isolate from the patient. To the best of our knowledge, this was the second report of a dual infection with Pasteurella species consisting of P. dagmatis and P. canis resulting from a dog-bite, followed by the first report of dual infections due to P. dagmatis and P. multocida in 1988. Our isolate finally identified as P. dagmatis was misidentified as P. pneumotripica by means of the Vitek 2 system. The species name "P. dagmatis" was not included in the database of the system. It is also important for routine clinical microbiology laboratories to know the limitation of the automated Vitek 2 system for the accurate identification of Pasteurella species especially P. dagmatis. It should be emphasized that there still exists much room for improvement in Vitek 2 system. Significant improvement of Vitek 2 system especially in the identification of Pasteurella species is urgently desired.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 051095978

Download citation: RISBibTeXText

PMID: 22112359

Related references

Dog bite wound infection by Pasteurella dagmatis misidentified as Pasteurella pneumotropica by automated system Vitek 2. Diagnostic Microbiology and Infectious Disease 65(3): 347-348, 2009

Reclassification of the Genus Pasteurella Trevisan 1887 on the Basis of Deoxyribonucleic Acid Homology, with Proposals for the New Species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis, and Pasteurella langaa. International Journal of Systematic Bacteriology 35(3): 309-322, 1985

Reclassification of the genus pasteurella on the basis of dna homology with proposals for the new species pasteurella dagmatis new species and pasteurella canis pasteurella stomatis new species pasteurella anatis new species pasteurella langaa new species. International Journal of Systematic Bacteriology 35(3): 309-322, 1985

Co-isolation of Pasteurella dagmatis and Pasteurella multocida from cat-bite wounds. European Journal of Clinical Microbiology and Infectious Diseases 7(2): 203-204, 1988

First report of Pasteurella canis infection in Japan resulting from dog bite. Nichidai Igaku Zasshi 56(7): 387-390, 1997

Revised description and classification of atypical isolates of Pasteurella multocida from bovine lungs based on genotypic characterization to include variants previously classified as biovar 2 of Pasteurella canis and Pasteurella avium. Microbiology 150(Pt 6): 1757-1767, 2004

Inst electron microscopic aspects of pasteurella animalium pasteurella multocida in different stages of incubation and conservation pasteurella avium pasteurella bovis. Archiva Veterinaria 2(2): 71-81, 1967

Wound infection caused by Pasteurella dagmatis following a cat scratch. Enfermedades Infecciosas Y Microbiologia Clinica 10(7): 438-439, 1992

Bacteremia due to Pasteurella dagmatis acquired from a dog bite, with a review of systemic infections and challenges in laboratory identification. Canadian Journal of Infectious Diseases and Medical Microbiology 26(5): 273-276, 2015

Identification of Pasteurella canis in a Soft Tissue Infection Caused by a Dog Bite: The First Report in Korea. Annals of Laboratory Medicine 36(6): 617-619, 2016

Long-term respiratory tract infection with canine-associated Pasteurella dagmatis and Neisseria canis in a patient with chronic bronchiectasis. Journal of Clinical Microbiology 43(8): 4272-4274, 2005

Antimicrobial minimal inhibitory concentration values of Pasteurella sp, Pasteurella haemolytica, and Pasteurella multocida of bovine and porcine origin using a customized Sensititre susceptibility testing system. Proceedings of annual meeting American Association of Veterinary Laboratory Diagnosticians: 7th) 265-276, 1984

PASTEURELLA MULTOCIDA INFECTION IN A DOG BITE WOUND: A Case Report. Medical Journal Armed Forces India 51(4): 288-289, 1995

Isolation of Pasteurella dagmatis after a tiger bite. Revista Espanola de Quimioterapia 27(4): 269-270, 2014

Actinobacillus rossii sp. nov., Actinobacillus seminis sp. nov., nom. rev., Pasteurella bettii sp. nov., Pasteurella lymphangitidis sp. nov., Pasteurella mairi sp. nov., and Pasteurella trehalosi sp. nov. International Journal of Systematic Bacteriology 40(2): 148-153, 1990