+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A comparative analysis of routine techniques: Reverse transcriptase polymerase chain reaction (RT-PCR) and five cell lines for detection of enteroviruses in stool specimens



A comparative analysis of routine techniques: Reverse transcriptase polymerase chain reaction (RT-PCR) and five cell lines for detection of enteroviruses in stool specimens



Iranian Journal of Microbiology 3(2): 75-79



Each year, Enteroviruses infect millions of people and cause different diseases. The agents are usually detected using cell culture. RD (Rhabdomyosarcoma) and L20B (L cells) are among the recommended cells by the World Health Organisation (WHO) for this purpose. Even though cell culture is the most common method used in diagnosing Enteroviruses in stool specimens, this particular method poses some problems, which include false positive or negative results, lack of a unique cell line for diagnosing all Enterovirus types in addition to being time consuming. For these reasons, an attempt was made to find better techniques of Enterovirus detection. RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) is a technique used in place of the cell culture method. In this study, the cell culture method was compared with RT-PCR for detection of Enteroviruses in stool specimens. First, the chloroform treated stool samples were inoculated onto five cell lines, including RD, L20B, Hep-2 (Human Epidermoid carcinoma cell line), Vero (Verde Reno) and GMK (Green Monkey Kidney). The results were then compared with data from Enterovirus detection using the RT-PCR technique. The difference between RT-PCR and cell culture results was significant. Enteroviruses were detected in 24% of specimens using RT-PCR while cell lines could isolate Enteroviruses in just 14.4% of the samples.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 051106179

Download citation: RISBibTeXText

PMID: 22347586


Related references

Detection of enteroviruses by reverse-transcriptase polymerase chain reaction in cell culture negative stool specimens of patients with acute flaccid paralysis. Journal of Virological Methods 142(1-2): 95-97, 2007

Detection of Norwalk virus in stool specimens by reverse transcriptase-polymerase chain reaction and nonradioactive oligoprobes. Journal of Clinical Microbiology 30(12): 3151-3157, 1992

Evaluation of a reverse transcriptase-polymerase chain reaction assay for the detection of enteroviruses in sludge. Abstracts of the General Meeting of the American Society for Microbiology 94: 416, 1994

Comparison of real-time reverse transcriptase-polymerase chain reaction and nested or commercial reverse transcriptase-polymerase chain reaction for the detection of hepatitis E virus particle in human serum (vol 56, pg 269, 2006). Diagnostic Microbiology and Infectious Disease 57(3): 353, 2007

Comparison of real-time reverse transcriptase-polymerase chain reaction and nested or commercial reverse transcriptase-polymerase chain reaction for the detection of hepatitis E virus particle in human serum. Diagnostic Microbiology and Infectious Disease 56(3): 269-274, 2006

Pelacakan kasus flu burung pada ayam dengan reverse transcriptase polymerase chain reaction* detection of avian influenza in chickens by reverse transcriptase polymerase chain reaction. 2013

Detection of human immunodeficiency virus type 2 by real time reverse transcriptase polymerase chain reaction and nested reverse transcriptase polymerase chain reaction. 2007

Detection of hepatitis C RNA in liver tissue by reverse-transcriptase polymerase chain reaction Analysis of sequential post-transplant biopsy specimens. Modern Pathology 12(1): 160A, 1999

Comparison of detection techniques for cytokine reverse transcriptase polymerase chain reaction; digoxigenin-labeled polymerase chain reaction permits sensitive detection of cytokine mRNA in rat heart allografts. Journal Of Immunological Methods. 217(1-2): 185-193,. 1, 1998

Comparative analysis of routine histology, immunohistochemistry, reverse transcriptase polymerase chain reaction, and fluorescence in situ hybridization in diagnosis of Ewing family of tumors. Archives of Pathology & Laboratory Medicine 130(12): 1813-1818, 2006

Detection and serotyping of rotaviruses in stool specimens by using reverse transcription and polymerase chain reaction amplification. Journal Of Medical Virology. 38(4): 292-297, 1992

Simple method of concentrating enteroviruses and hepatitis A virus from sewage and ocean water for rapid detection by reverse transcriptase-polymerase chain reaction. Applied and Environmental Microbiology 59(10): 3488-3491, 1993

Detection of tumor cells in peripheral blood samples from patients with germ cell tumors using immunocytochemical and reverse transcriptase-polymerase chain reaction techniques. Bone Marrow Transplantation 22(8): 771-775, 1998

Rapid detection and typing of dengue viruses from serum specimens by reverse transcriptase-polymerase chain reaction. Zhongguo Meijieshengwuxue ji Kongzhi Zazhi = Chinese Journal of Vector Biology and Control 8(6): 447-451, 1997

Increased detection of rotavirus using a real time reverse transcription-polymerase chain reaction (RT-PCR) assay in stool specimens from children with diarrhea. Journal of Medical Virology 72(3): 496-501, 2004