+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A modified matrix perfusion-microcarrier bead cell culture system. II. Production of human (beta) interferon in a matrix perfusion system

A modified matrix perfusion-microcarrier bead cell culture system. II. Production of human (beta) interferon in a matrix perfusion system

Biotechnology and Bioengineering 26(5): 508-512

Addition of microcarrier beads to a matrix perfusion cell culture system allowed growth of anchorage dependent human foreskin fibroblasts which would not grow in the culture units alone. The utility of the system for collection of cellular products was demonstrated by the induction and harvesting of human (beta) interferon. Interferon production was highest in perfusion cultures when medium was circulated throughout the induction and when inducer containing 100 mug/mL polyriboinosinic: polyribocytidylic acid was placed directly in contact with cells in the extracapillary space. These conditions provided 4-to-10-fold greater interferon yields per cell, and approximately 12-fold increases per vessel, than monolayer cultures. Perfusion grown cells produced interferon at a maximal level for 20 h postinduction compared to approximately 2 h for monolayer grown cells, thus giving a higher total yield of interferon. Other procedures increasing the efficiency of the system included priming with 50 U/mL interferon standard, reinduction of cells, use of antibiotic free medium, reduced serum concentrations, and in vitro aging of the cells.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 051165248

Download citation: RISBibTeXText

PMID: 18553347

DOI: 10.1002/bit.260260516

Related references

A modified matrix perfusion-microcarrier bead cell culture system. I. Adaptation of the matrix perfusion system for growth of human foreskin fibroblasts. Biotechnology and Bioengineering 26(5): 503-507, 1984

Human fibroblast interferon production in a matrix perfusion micro carrier bead system. Abstracts of the Annual Meeting of the American Society for Microbiology 82: ABSTRACT T20, 1982

Matrix perfusion cell culture system for cancer chemo therapeutic agents. In Vitro (rockville): 175-176, 1979

Carcino embryonic antigen production by human colo rectal tumor cells in a matrix perfusion system. In Vitro 14(4): 335, 1978

Human periosteal-derived cell expansion in a perfusion bioreactor system: proliferation, differentiation and extracellular matrix formation. Journal of Tissue Engineering and Regenerative Medicine 11(2): 519-530, 2017

Carcinoembryonic Antigen Production by Human Colorectal Adenocarcinoma Cells in Matrix-Perfusion Culture. In Vitro 16(2): 113-118, 1980

Carcinoembryonic antigen production by human colorectal adenocarcinoma cells in matrix-perfusion culture. In Vitro 16(2): 113-118, 1980

Organotypical engineering of differentiated composite-skin equivalents of human keratinocytes in a collagen-GAG matrix (INTEGRA Artificial Skin) in a perfusion culture system. Langenbeck's Archives of Surgery 386(5): 357-363, 2001

Perfusion culture system: Synovial fibroblasts modulate articular chondrocyte matrix synthesis in vitro. Tissue and Cell 42(3): 151-157, 2010

Production of rat stem cell factor from BRL cells by microcarrier perfusion culture. Annals of the new York Academy of Sciences 665: 371-379, 1992

Production of rat serum cell factor from BRL cells by microcarrier perfusion culture. (see Book Title) [Author] Pedersen, H , R Mutharasan and D DiBiasio (Ed ) Annals of the New York Academy of Sciences, Vol 665 Biochemical engineering; Seventh Biochemical Engineering Conference: Cellular and Reactor Engineering, Santa Barbara, California, USA, March 3-8, 1991 x+450p New York Academy of Sciences: New York, New York, USA Illus ISBN 0-89766-736-0(paper); ISBN 0-89766-735-2(cloth): 371-379, 1992

Effect of glucocorticoid hormones on growth of human fibroblast cells and interferon production in a microcarrier culture system. Cell Structure and Function 12(6): 509-517, 1987

Perfusion culture using microcarrier for the production of Varicella-Zoster virus in human embryonic lung cells. Biotechnology Letters 21(2): 129-133, 1999

The changes of extracellular matrix in adult degenerative nucleus pulposus cells with stiring microcarrier culture system in vitro. Zhonghua Wai Ke Za Zhi 51(5): 432-436, 2013

Successful scale-up of human embryonic stem cell production in a stirred microcarrier culture system. Brazilian Journal of Medical and Biological Research 42(6): 515-522, 2009