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A reverse transcriptase stop assay revealed diverse quadruplex formations in UTRs in mRNA

A reverse transcriptase stop assay revealed diverse quadruplex formations in UTRs in mRNA

Bioorganic and Medicinal Chemistry Letters 20(7): 2350-2353

Here, we developed a reverse transcriptase based method (RTase stop assay) to characterize quadruplex formations in guanine-rich RNAs with high sensitivity and specificity. By using the RTase stop assay, we also revealed a plausible structural polymorphism in biologically important RNAs. The RTase stop assay would provide helpful insight into RNA quadruplex structures and functions, together with other analytical methods, including various footprinting techniques.

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Accession: 051240367

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PMID: 20206514

DOI: 10.1016/j.bmcl.2010.01.158

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