+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator



Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator



Plos One 7(6): E40003



In this report, a Ti:Sapphire oscillator was utilized to realize synchronization-free stimulated emission depletion (STED) microscopy. With pump power of 4.6 W and sample irradiance of 310 mW, we achieved super-resolution as high as 71 nm. With synchronization-free STED, we imaged 200 nm nanospheres as well as all three cytoskeletal elements (microtubules, intermediate filaments, and actin filaments), clearly demonstrating the resolving power of synchronization-free STED over conventional diffraction limited imaging. It also allowed us to discover that, Dylight 650, exhibits improved performance over ATTO647N, a fluorophore frequently used in STED. Furthermore, we applied synchronization-free STED to image fluorescently-labeled intracellular viral RNA granules, which otherwise cannot be differentiated by confocal microscopy. Thanks to the widely available Ti:Sapphire oscillators in multiphoton imaging system, this work suggests easier access to setup super-resolution microscope via the synchronization-free STED.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 051332758

Download citation: RISBibTeXText

PMID: 22761944

DOI: 10.1371/journal.pone.0040003


Related references

Increasing fluorescence lifetime for resolution improvement in STED nanoscopy. Journal of Biophotonics 2018: E201800315, 2018

Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution. Biophysical Journal 105(1): L01-L03, 2014

Low-Saturation-Intensity, High-Photostability, and High-Resolution STED Nanoscopy Assisted by CsPbBr 3 Quantum Dots. Advanced Materials 30(23): E1800167, 2018

Investigation on achieving super-resolution by solid immersion lens based STED microscopy. Optics Express 25(14): 16629-16642, 2017

Sw 2Pe-Sted Nanoscopy. Biophysical Journal 104(2): 534a-535a, 2013

Adaptive-illumination STED nanoscopy. Proceedings of the National Academy of Sciences of the United States of America 114(37): 9797-9802, 2017

STED nanoscopy: a glimpse into the future. Cell and Tissue Research 360(1): 143-150, 2015

Parallelized STED fluorescence nanoscopy. Optics Express 19(24): 23716-23726, 2012

STED nanoscopy with mass-produced laser diodes. Optics Express 19(9): 8066-8072, 2012

Coma aberrations in combined two- and three-dimensional STED nanoscopy. Optics Letters 41(15): 3631-3634, 2016

Large parallelization of STED nanoscopy using optical lattices. Optics Express 22(5): 5581-5589, 2014

Choosing dyes for cw-STED nanoscopy using self-assembled nanorulers. Physical Chemistry Chemical Physics 16(15): 6990-6996, 2014

Dual-label STED nanoscopy of living cells using photochromism. Nano Letters 11(9): 3970-3973, 2012

Double-helix enhanced axial localization in STED nanoscopy. Optics Express 21(25): 30984-30992, 2014

Neuro at the Nanoscale: Diffraction-Unlimited Imaging with STED Nanoscopy. Journal of Histochemistry and Cytochemistry 63(12): 897-907, 2016