+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Activation of the jasmonic acid pathway by depletion of the hydroperoxide lyase OsHPL3 reveals crosstalk between the HPL and AOS branches of the oxylipin pathway in rice



Activation of the jasmonic acid pathway by depletion of the hydroperoxide lyase OsHPL3 reveals crosstalk between the HPL and AOS branches of the oxylipin pathway in rice



Plos One 7(11): E50089



The allene oxide synthase (AOS) and hydroperoxide lyase (HPL) branches of the oxylipin pathway, which underlie the production of jasmonates and aldehydes, respectively, function in plant responses to a range of stresses. Regulatory crosstalk has been proposed to exist between these two signaling branches; however, there is no direct evidence of this. Here, we identified and characterized a jasmonic acid (JA) overproduction mutant, cea62, by screening a rice T-DNA insertion mutant library for lineages that constitutively express the AOS gene. Map-based cloning was used to identify the underlying gene as hydroperoxide lyase OsHPL3. HPL3 expression and the enzyme activity of its product, (E)-2-hexenal, were depleted in the cea62 mutant, which resulted in the dramatic overproduction of JA, the activation of JA signaling, and the emergence of the lesion mimic phenotype. A time-course analysis of lesion formation and of the induction of defense responsive genes in the cea62 mutant revealed that the activation of JA biosynthesis and signaling in cea62 was regulated in a developmental manner, as was OsHPL3 activity in the wild-type plant. Microarray analysis showed that the JA-governed defense response was greatly activated in cea62 and this plant exhibited enhanced resistance to the T1 strain of the bacterial blight pathogen Xanthomonasoryzaepvoryzae (Xoo). The wounding response was attenuated in cea62 plants during the early stages of development, but partially recovered when JA levels were elevated during the later stages. In contrast, the wounding response was not altered during the different developmental stages of wild-type plants. These findings suggest that these two branches of the oxylipin pathway exhibit crosstalk with regards to biosynthesis and signaling and cooperate with each other to function in diverse stress responses.

(PDF emailed within 0-6 h: $19.90)

Accession: 051344859

Download citation: RISBibTeXText

PMID: 23209649

DOI: 10.1371/journal.pone.0050089


Related references

The rice hydroperoxide lyase OsHPL3 functions in defense responses by modulating the oxylipin pathway. Plant Journal 71(5): 763-775, 2013

Green leaf volatiles: hydroperoxide lyase pathway of oxylipin metabolism. Current Opinion in Plant Biology 9(3): 274-280, 2006

The hydroperoxide lyase branch of the oxylipin pathway protects against photoinhibition of photosynthesis. Planta 245(6): 1179-1192, 2017

Silencing of hydroperoxide lyase and allene oxide synthase reveals substrate and defense signaling crosstalk in Nicotiana attenuata. Plant Journal 40(1): 35-46, 2004

α-linolenic acid concentration and not wounding per se is the key regulator of octadecanoid (oxylipin) pathway activity in rice (Oryza sativa L.) leaves. Plant Physiology and Biochemistry 83: 117-125, 2015

Tomato allene oxide synthase and fatty acid hydroperoxide lyase, two cytochrome P450s involved in oxylipin metabolism, are targeted to different membranes of chloroplast envelope. Plant Physiology 125(1): 306-317, 2001

Oxylipin pathway in rice and Arabidopsis. Journal of Integrative Plant Biology 49(1): 43-51, 2007

Transcript profile analysis reveals important roles of jasmonic acid signalling pathway in the response of sweet potato to salt stress. Scientific Reports 7: 40819, 2017

Elevated CO 2 differentially affects tobacco and rice defense against lepidopteran larvae via the jasmonic acid signaling pathway. Journal of Integrative Plant Biology 60(5): 412-431, 2018

Activation of the jasmonic acid plant defence pathway alters the composition of rhizosphere bacterial communities. Plos One 8(2): E56457, 2013

A dual mechanism of activation of the Sonic Hedgehog pathway in anaplastic thyroid cancer: crosstalk with RAS-BRAF-MEK pathway and ligand secretion by tumor stroma. Oncotarget 9(4): 4496-4510, 2018

Riboflavin-induced resistance against rice sheath blight functions through the potentiation of lignin formation and jasmonic acid signalling pathway. Communications in Agricultural and Applied Biological Sciences 72(2): 309-313, 2008

Alterations of mitochondrial protein assembly and jasmonic acid biosynthesis pathway in Honglian (HL)-type cytoplasmic male sterility rice. Journal of Biological Chemistry 287(47): 40051-40060, 2013

Global gene expression profiling during Medicago truncatula-Phymatotrichopsis omnivora interaction reveals a role for jasmonic acid, ethylene, and the flavonoid pathway in disease development. Molecular Plant-Microbe Interactions 22(1): 7-17, 2008

A novel rice PR10 protein, RSOsPR10, specifically induced in roots by biotic and abiotic stresses, possibly via the jasmonic acid signaling pathway. Plant and cell physiology 45(5): 550-559, 2004