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Alternatives to retroorbital blood collection in hispid cotton rats (Sigmodon hispidus)


Alternatives to retroorbital blood collection in hispid cotton rats (Sigmodon hispidus)



Journal of the American Association for Laboratory Animal Science: Jaalas 51(2): 239-245



ISSN/ISBN: 1559-6109

PMID: 22776125

Cotton rats (Sigmodon hispidus) are a valuable animal model for many human viral diseases, including polio virus, measles virus, respiratory syncytial virus, and herpes simplex virus. Although cotton rats have been used in research since 1939, few publications address handling and sampling techniques for this species, and the retroorbital sinus remains the recommended blood sampling site. Here we assessed blood sampling methods that are currently used in other species and a novel subzygomatic sampling site for their use in S. hispidus. The subzygomatic approach accesses a venous sinus that possibly is unique to this species and that lies just below the zygomatic arch of the maxilla and deep to the masseter muscle. We report that both the novel subzygomatic approach and the sublingual vein method can be used effectively in cotton rats.

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Accession: 051448737

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