+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Analysis of expressed sequence tags in Brassica napus cotyledons damaged by crucifer flea beetle feeding

Analysis of expressed sequence tags in Brassica napus cotyledons damaged by crucifer flea beetle feeding

Genome 55(2): 118-133

The molecular basis of canola ( Brassica napus L.) susceptibility to the crucifer flea beetle (FB, Phyllotreta cruciferae Goeze) was investigated by comparing transcript representation in FB-damaged and undamaged cotyledons. The B. napus cotyledon transcriptome increased and diversified substantially after FB feeding damage. Twenty-two genes encoding proteins with unknown function, six encoding proteins involved in signaling, and a gene encoding a B-box zinc finger transcription factor were moderately or strongly changed in representation with FB feeding damage. Zinc finger and calcium-dependent genes formed the largest portion of transcription factors and signaling factors with changes in representation. Six genes with unknown function, one transcription factor, and one signaling gene specific to the FB-damaged library were co-represented in a FB-damaged leaf library. Out of 188 transcription factor and signaling gene families screened for "early" expression changes, 16 showed changes in expression within 8 h. Four of these early factors were zinc finger genes with representation only in the FB-damaged cotyledon. These genes are now available to test their potential at initiating or specifying cotyledon responses to crucifer FB feeding.

(PDF emailed within 0-6 h: $19.90)

Accession: 051522138

Download citation: RISBibTeXText

PMID: 22276855

DOI: 10.1139/g11-083

Related references

Susceptibility of inbred lines of oilseed rape, Brassica napus, to feeding damage by the crucifer flea beetle, Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae), and its inheritance. Canadian Journal of Plant Science 73(2): 615-623, 1993

The biochemical composition and transcriptome of cotyledons from Brassica napus lines expressing the AtGL3 transcription factor and exhibiting reduced flea beetle feeding. Bmc Plant Biology 18(1): 64, 2018

Glucosinolate levels in cotyledons of mustard,Brassica juncea L. and rape,B. napus L. do not determine feeding rates of flea beetle,Phyllotreta cruciferae (Goeze). Journal of Chemical Ecology 16(9): 2735-2746, 1990

Effects of simulated and flea beetle injury to cotyledons on growth of drought-stressed oilseed rape, Brassica napus L. Canadian Journal of Plant Science 77(3): 475-481, 1997

Expressed sequence tags from the defense of Brassica napus to Leptosphaeria maculans. Molecular Plant Pathology On line: ( 0301 Fristensky), 1999

Mining expressed sequence tags of rapeseed (Brassica napus L.) to predict the drought responsive regulatory network. Physiology and Molecular Biology of Plants 21(3): 329-340, 2015

Generation of expressed sequence tags of random root cDNA clones of Brassica napus by single-run partial sequencing. Plant Physiology 103(2): 359-370, 1993

Development and application of single nucleotide polymorphism markers in the polyploid Brassica napus by 454 sequencing of expressed sequence tags. Plant Breeding 131(2): 293-299, 2012

Responses of the crucifer flea beetle to Brassica volatiles in an olfactometer. Environmental Entomology 38(5): 1467-1479, 2009

Analysis of expressed sequence tags of the water flea Daphnia magna. Genome 48(4): 606-609, 2005

Response of four brassica seed crop species to attack by the crucifer flea beetle, Phyllotreta cruciferae. Canadian Journal of Plant Science 57(3): 987-989, 1977

Analysis and comparison of a set of expressed sequence tags of the parthenogenetic water flea Daphnia carinata. Molecular Genetics and Genomics 282(2): 197-203, 2009

Analysis of expressed sequence tags from Brassica rapa L. ssp. pekinensis. Molecules and Cells 10(4): 399-404, 2000

Generation of 919 expressed sequence tags from immature flower buds and gene expression analysis using expressed sequence tags in the model plant Lotus japonicus. Genes & Genetic Systems 77(4): 277-282, 2002

Crucifer-feeding flea beetle dispersal and statistics of directional data. Environmental Entomology, 125: 1380-1383, 1983