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Analysis of protein structure by cross-linking combined with mass spectrometry

Analysis of protein structure by cross-linking combined with mass spectrometry

Methods in Molecular Biology 1156: 447-463

Cross-linking combined with mass spectrometry is a powerful technique to study protein structure. Here, we present an optimized protocol for the preparation, processing, and analysis of a protein sample cross-linked with isotopically coded, affinity-enrichable, and CID-cleavable cross-linker CyanurBiotinDimercaptoPropionylSuccinimide using LC/ESI-MS/MS on a Thermo Scientific Orbitrap mass spectrometer.

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Accession: 051527213

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PMID: 24792007

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