+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Anti-SARS coronavirus 3C-like protease effects of Rheum palmatum L. extracts



Anti-SARS coronavirus 3C-like protease effects of Rheum palmatum L. extracts



Bioscience Trends 3(4): 124-126



The present study aims to clarify the inhibitive effect of the compounds from Rheum palmatum L. on the SARS-3CL protease. The SARS-CoV 3CL gene was amplified from RNA of the SARS virus by PCR. The SARS-CoV 3CL protease was purified from a colon bacillus recombinant. Drugs and 3CL protease were incubated together. The inhibition rate and IC(50) were calculated based on absorbance. Components from the Rheum palmatum L. had a high level of anti-SARS-CoV 3CL protease activity. The IC(50) was 13.76 +/- 0.03 microg/mL and the inhibition rate was up to 96%. In conclusion, extracts from Rheum palmatum L. have a high level of inhibitory activity against 3CL protease, suggesting that extracts from Rheum palmatum L. may represent a potential therapeutic for SARS.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 051565819

Download citation: RISBibTeXText

PMID: 20103835


Related references

Characterization of trans- and cis-cleavage activity of the SARS coronavirus 3CLpro protease: basis for the in vitro screening of anti-SARS drugs. Febs Letters 574(1-3): 131-137, 2004

Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds. Antiviral Research 68(1): 36-42, 2005

Development of a red-shifted fluorescence-based assay for SARS-coronavirus 3CL protease: identification of a novel class of anti-SARS agents from the tropical marine sponge Axinella corrugata. Biological Chemistry 387(8): 1063-1074, 2006

Optimization of chloroplast microsatellite PCR conditions and primer screening for endangered Rheum officinale, Rheum palmatum, and Rheum tanguticum. Genetics and Molecular Research 13(3): 5787-5794, 2014

Anti-proliferative effects of estrogen receptor-modulating compounds isolated from Rheum palmatum. Archives of Pharmacal Research 31(6): 722-726, 2008

Anti-metastatic effects of Rheum Palmatum L. extract in human MDA-MB-231 breast cancer cells. Environmental Toxicology and Pharmacology 40(1): 30-38, 2015

Effect of different extracts of Rheum palmatum on trypsin activity. Zhong Yao Tong Bao 10(4): 41, 1985

The laxative action of differently prepared dry rheum palmatum d extracts. Herba Polonica 14(1-2): 38-44, 1968

Anti-SARS virus antibody responses against human SARS-associated coronavirus and animal SARS-associated coronavirus-like virus. Chinese Medical Journal 117(11): 1723-1725, 2004

Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia. Journal of Clinical Microbiology 43(7): 3054-3058, 2005

Analysis of anthraquinones in rhubarb (Rheum palmatum and Rheum officinale) by supercritical fluid chromatography. Talanta 144: 1239-1244, 2015

Morphology and anatomy of underground organs of Chinese Rheum (Rheum palmatum L.) experimentally cultivated in the Belgrade area. 2d report. Glas. Srpska Akademija Nauka i Umetnosti. Odeljenje Medicinskih Nauka 1967(20): 93-99, 1967

Genetic variation in Rheum palmatum and Rheum tanguticum (Polygonaceae), two medicinally and endemic species in China using ISSR markers. Plos one 7(12): E51667, 2012

An efficient method for the synthesis of peptide aldehyde libraries employed in the discovery of reversible SARS coronavirus main protease (SARS-CoV Mpro) inhibitors. Chembiochem 7(7): 1048-1055, 2006

Antiviral activities of extracts isolated from Terminalis chebula Retz., Sanguisorba officinalis L., Rubus coreanus Miq. and Rheum palmatum L. against hepatitis B virus. Phytotherapy Research 15(8): 718-720, 2001