+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Antimicrobial susceptibilities of coagulase-negative staphylococci (CNS) and streptococci from bovine subclinical mastitis cases



Antimicrobial susceptibilities of coagulase-negative staphylococci (CNS) and streptococci from bovine subclinical mastitis cases



Journal of Microbiology 48(3): 267-274



The prevalence and antimicrobial susceptibilities of Staphylococci and Streptococci were assessed from subclinical mastitis cases. One hundred Coagulase-Negative Staphylococci (CNS) and 34 Streptococci were identified. The most frequently isolated species were Staphylococcus haemolyticus (27%) and Staphylococcus simulans (24%). Susceptible CNS species revealed the highest resistance to penicillin G (58%), ampicillin (48%), neomycin (20%), and oleandomycin (14%). CNS methicillin resistance rates within 82 isolates were 21.95% and 1.22% by disk diffusion and PCR methods, respectively. These results suggested the disk diffusion method was more prone to yield false positives. Partial sequencing of the 16S rRNA region from the mecA carrying isolate (S. haemolyticus) was homologous with S. haemolyticus sequences/accessions obtained from GenBank. However, the mecA gene sequence from this isolate was more closely allied with the S. aureus mecA gene of human origins. Identical sequence data was acquired from the National Center for Biotechnology Information (NCBI) database, suggesting horizontal gene transfer between the two species. CNS beta-lactamase activity within 81 isolates was 29.63%. The most frequently isolated Streptococcus species were S. uberis (52%) and S. agalactiae (15%). Oleandomycin was the least effective antimicrobial agent on these isolates with 59% susceptibility. Results indicated that CNS and Streptococci exhibited various antimicrobial resistance responses. Consequently, isolation and identification of udder pathogens in herds suffering from subclinical agents is essential to select the most effective antimicrobial agent. Moreover, multiple resistance features of methicillin resistant (MR) isolates should be considered during antimicrobial susceptibility tests.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 051586130

Download citation: RISBibTeXText

PMID: 20571942

DOI: 10.1007/s12275-010-9373-9


Related references

Macrolide-lincosamide resistance among coagulase-negative staphylococci from cases of bovine subclinical mastitis. 2006

Antimicrobial resistance of coagulase-negative staphylococci from bovine subclinical mastitis with particular reference to macrolide-lincosamide resistance phenotypes and genotypes. Journal of Antimicrobial ChemoTherapy 57(5): 966-969, 2006

Bovine subclinical mastitis caused by different types of coagulase-negative staphylococci. Journal of Dairy Science 92(10): 4962-4970, 2009

Identification of coagulase negative staphylococci causing subclinical mastitis and their antimicrobial resistance patterns. Thai Journal of Veterinary Medicine 30(2): 23-33, 2000

Classification of coagulase-negative staphylococci isolated from bovine clinical and subclinical mastitis. Veterinary Microbiology 27(2): 151-158, 1991

Resistance and plasmid profile of coagulase negative staphylococci isolate from bovine subclinical mastitis in Brazil. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 47(4): 487-497, 1995

Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis in Argentina. Journal of dairy science02 85(8): 1913-1917, 2002

Characterization of coagulase negative staphylococci from cases of subclinical mastitis in dairy cattle in Kampala, Uganda. Irish Veterinary Journal 67(1): 12, 2014

Comparison of a commercialized phenotyping system, antimicrobial susceptibility testing, and tuf gene sequence-based genotyping for species-level identification of coagulase-negative staphylococci isolated from cases of bovine mastitis. Veterinary Microbiology 134(3-4): 327-333, 2008

Genetic characterization of antimicrobial resistance in coagulase-negative staphylococci from bovine mastitis milk. Journal of Dairy Science 96(4): 2247-2257, 2013

Antimicrobial activity of marine sponges against coagulase-negative staphylococci isolated from bovine mastitis. Veterinary Microbiology 155(2-4): 362-368, 2013

Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis between 2003 and 2008 in Korea. Journal of Microbiology and Biotechnology 20(10): 1446-1449, 2010

Differentiation of coagulase negative staphylococci (CNS) from cases of bovine mastitis and their antibiotic sensitivity. Journal of Veterinary Medicine, Japan 51(11): 893-896, 1998

Antimicrobial sensitivity and beta -lactamase production of Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitis. Veterinaria Mexico 22(3): 290-294, 1991

Identification and antimicrobial susceptibility of Staphylococcus aureus and coagulase negative staphylococci from bovine mastitis in the Aydn Region of Turkey. Turk Veterinerlik ve Hayvanclk Dergisi 29(3): 791-796, 2005