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Application of a fast sorting algorithm to the assignment of mass spectrometric cross-linking data

Application of a fast sorting algorithm to the assignment of mass spectrometric cross-linking data

Proteomics 14(17-18): 1987-1989

Cross-linking combined with MS involves enzymatic digestion of cross-linked proteins and identifying cross-linked peptides. Assignment of cross-linked peptide masses requires a search of all possible binary combinations of peptides from the cross-linked proteins' sequences, which becomes impractical with increasing complexity of the protein system and/or if digestion enzyme specificity is relaxed. Here, we describe the application of a fast sorting algorithm to search large sequence databases for cross-linked peptide assignments based on mass. This same algorithm has been used previously for assigning disulfide-bridged peptides (Choi et al., ), but has not previously been applied to cross-linking studies.

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Accession: 051607236

Download citation: RISBibTeXText

PMID: 24895266

DOI: 10.1002/pmic.201300486

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