+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts



Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts



Journal of Biotechnology 182-183: 68-73



The use of antibiotic resistance markers in the commercial application of genetically modified microorganisms is limited due to restrictions on the release of antibiotics and their resistance genes to the environment. To avoid contamination by other microorganisms, the development of a dominant selection marker with low environmental risks is still needed. Here we demonstrated a new selection system for Schizosaccharomyces pombe and Saccharomyces cerevisiae using a bacterial phosphite dehydrogenase gene (ptxD). A Sz. pombe transformant carrying ptxD under a strong promoter or on a multicopy plasmid grew on a minimal medium containing phosphite (Pt) as a sole source of phosphorus. To adapt this system to S. cerevisiae strains, codon optimization of ptxD was necessary. The codon-optimized ptxD system appeared effective in not only laboratorial but also industrial S. cerevisiae strains that are diploid or polyploid. Since Pt is a safe and inexpensive chemical, ptxD could be used as a novel dominant selection marker applicable on an industrial scale.

(PDF emailed within 0-6 h: $19.90)

Accession: 051607580

Download citation: RISBibTeXText

PMID: 24786825

DOI: 10.1016/j.jbiotec.2014.04.012



Related references

Transformation system for prototrophic industrial yeasts using the AUR1 gene as a dominant selection marker. FEBS Letters 425(1): 117-122, March 20, 1998

Cycloheximide resistance a dominant selection marker for transformation of laboratory and industrial yeasts. Organizing Committee Of Biotec-90 From Genes to Bioproducts; 3rd Spanish Congress Of Biotechnology, Murcia, Spain, June Xxiv+409p Promociones Y Publicaciones Universitarias, S A : Barcelona, Spain; Dm: Murcia, Spain Illus Paper 113-116, 1990

Dominant selectable marker for gene transformation and disruption in yeasts. Official Gazette of the United States Patent & Trademark Office Patents 1270(2), May 13, 2003

A SCAR marker applicable in marker assisted selection of a dominant male sterility gene in cabbage. Acta Horticulturae Sinica 27(2): 143-144, 2000

Development of a co?dominant DNA marker linked to the gene lentus conferring reduced pod shattering for marker?assisted selection in narrow?leafed lupin Lupinus angustifolius breeding. Plant Breeding 131(4), 2012

A novel dominant selectable system for the selection of transgenic plants under in vitro and greenhouse conditions based on phosphite metabolism. Plant Biotechnology Journal 11(4): 516-525, 2013

D-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants. Febs Letters 586(1): 36-40, 2012

P450 BM3 fused to phosphite dehydrogenase allows phosphite-driven selective oxidations. Applied Microbiology and Biotechnology (): -, 2016

The Claviceps purpurea glyceraldehyde-3-phosphate dehydrogenase gene: cloning, characterization, and use for the improvement of a dominant selection system. Current Genetics 25(2): 101-106, 1994

Application of a new co-dominant PCR marker of high molecular weight glutenin gene Glu-D1d in wheat breeding. Jiangsu Journal Of Agricultural Sciences: 4, 394-399, 2008

Development of a locus-specific, co-dominant SCAR marker for assisted-selection of the Sw-5 gene cluster in a wide range of tomato accessions. Molecular Breeding: 1, 133-142, 2010

A convenient dominant selection marker for gene transfer in industrial strains of Saccharomyces yeast: SMRI encoded resistance to the herbicide sulfometuron methyl. Journal of the Institute of Brewing, UK 94(2): 93-97, 1988

Pyrithiamine resistance gene (ptrA) of Aspergillus oryzae: cloning, characterization and application as a dominant selectable marker for transformation. Bioscience, Biotechnology, and Biochemistry 64(7): 1416-1421, 2000