+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Application of a ready-to-use calcium ionophore increases rates of fertilization and pregnancy in severe male factor infertility

Application of a ready-to-use calcium ionophore increases rates of fertilization and pregnancy in severe male factor infertility

Fertility and Sterility 98(6): 1432-1437

To analyze whether a ready-to-use calcium ionophore improves outcomes, from fertilization to live birth, in patients with severe male factor infertility. Artificial oocyte activation offered to applicable patients over a 20-month period. Specialized in vitro fertilization (IVF) centers in Austria and Germany. Twenty-nine azoospermic and 37 cryptozoospermic men. Mature oocytes treated with a ready-to-use Ca(2+)-ionophore (GM508 Cult-Active) immediately after intracytoplasmic sperm injection (ICSI). Rates of fertilization, implantation, clinical pregnancy, and live birth. Patients had had 88 previous cycles without artificial activation that resulted in a fertilization rate of 34.7%, 79 transfers (89.8%), and 5 pregnancies, which all spontaneously aborted except one. After artificial oocyte activation, the fertilization rate was 56.9%. In terms of fertilization rate, both azoospermic (64.4%) and cryptozoospermic (48.4%) men statistically significantly benefited from use of the ionophore. In 73 transfer cycles, positive β-human chorionic gonadotropin levels were observed in 34 cases (46.6%) and 29 cycles (39.7%) that ended with a clinical pregnancy. The corresponding implantation rate was 33.3%. Four spontaneous abortions occurred (11.8%), and 32 healthy children were born. This is the first prospective multicenter study on artificial oocyte activation in severe male factor infertility. Present data indicate that a ready-to-use calcium ionophore can yield high fertilization and pregnancy rates for this particular subgroup. In addition to fertilization failure after ICSI, severe male factor infertility is an additional area for application of artificial oocyte activation.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 051607636

Download citation: RISBibTeXText

PMID: 22921909

DOI: 10.1016/j.fertnstert.2012.07.1134

Related references

Sperm treatment with extracellular ATP increases fertilization rates in in-vitro fertilization for male factor infertility. Human Reproduction 14(3): 694-697, 1999

Human Fallopian tube epithelial cell co-culture increases fertilization rates in male factor infertility but not in tubal or unexplained infertility. Human Reproduction 12(6): 1253-1258, 1997

Intracytoplasmic sperm injection: achievement of high pregnancy rates in couples with severe male factor infertility is dependent primarily upon female and not male factors. Fertility and Sterility 64(5): 977-981, 1995

Does previous salpingectomy improve implantation and pregnancy rates in patients with severe tubal factor infertility who are undergoing in vitro fertilization? A pilot prospective randomized study. Fertility & Sterility. 69(6): 1020-1025, E, 1998

Activation of human oocytes using calcium ionophore after ICSI increases fertilization and number of embryos for couples with low ICSI fertilization rates. Human Reproduction 14(ABSTR BOOK 1): 32, 1999

Experience with assisted fertilization in severe male factor infertility and unexplained failed fertilization in vitro. Human Reproduction 9(4): 680-683, 1994

Successful pregnancy after SrCl2 oocyte activation in couples with repeated low fertilization rates following calcium ionophore treatment. Systems Biology in Reproductive Medicine 60(3): 177-182, 2014

Pregnancy after in vitro fertilization and embryo transfer in severe immune male infertility. Andrologia 21(6): 516-518, 1989

Can calcium ionophore "use" in patients with diminished ovarian reserve increase fertilization and pregnancy rates? A randomized, controlled study. Fertility and Sterility 104(5): 1168-1174, 2015

In vitro fertilization outcome in the presence of severe male factor infertility. Fertility and Sterility 63(5): 1032-1037, 1995

The relation of male factor infertility and human egg fertilization and embryo division rates. Journal of In Vitro Fertilization & Embryo Transfer 3(3): 155, 1986

Intrauterine insemination with husband's sperm: comparison of pregnancy rates in couples with cervical factor, male factor, immunological factor and idiopathic infertility. Australian and new Zealand Journal of Obstetrics and Gynaecology 33(2): 183-186, 1993

Platelet-activating factor significantly enhances intrauterine insemination pregnancy rates in non-male factor infertility. Fertility and Sterility 82(1): 52-56, 2004

Artificial oocyte activation with calcium ionophore allowed fertilization and pregnancy in a couple with long-term unexplained infertility where the female partner had diminished EGG reserve and failure to fertilize oocytes despite intracytoplasmic sperm injection. Clinical and Experimental Obstetrics and Gynecology 37(4): 263-265, 2010