+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Application of alternative fixatives to formalin in diagnostic pathology



Application of alternative fixatives to formalin in diagnostic pathology



European Journal of Histochemistry 56(2): E12



Fixation is a critical step in the preparation of tissues for histopathology. The objective of this study was to investigate the effects of different fixatives vs formalin on proteins and DNA, and to evaluate alternative fixation for morphological diagnosis and nucleic acid preservation for molecular methods. Forty tissues were fixed for 24 h with six different fixatives: the gold standard fixative formalin, the historical fixatives Bouin and Hollande, and the alternative fixatives Greenfix, UPM and CyMol. Tissues were stained (Haematoxylin-Eosin, Periodic Acid Schiff, Trichromic, Alcian-blue, High Iron Diamine), and their antigenicity was determined by immunohistochemistry (performed with PAN-CK, CD31, Ki-67, S100, CD68, AML antibodies). DNA extraction, KRAS sequencing, FISH for CEP-17, and flow cytometry analysis of nuclear DNA content were applied. For cell morphology the alternative fixatives (Greenfix, UPM, CyMol) were equivalent to formalin. As expected, Hollande proved the best fixative for morphology. The morphology obtained with Bouin was comparable to that with formalin. Hollande was the best fixative for histochemistry. Bouin proved equivalent to formalin. The alternative fixatives were equivalent to formalin, although with greater variability in haematoxylin-eosin staining. It proved possible to obtain immunohistochemical staining largely equivalent to that following formalin-fixation with the following fixatives: Greenfix, Hollande, UPM and CyMol. The tissues fixed in Bouin did not provide results comparable to those obtained with formalin. The DNA extracted from samples fixed with alternative fixatives was found to be suitable for molecular analysis.

(PDF emailed within 0-6 h: $19.90)

Accession: 051607989

Download citation: RISBibTeXText

PMID: 22688293

DOI: 10.4081/ejh.2012.12


Related references

Histomorphologic assessment of formalin substitute fixatives for diagnostic surgical pathology. Archives of Pathology and Laboratory Medicine 129(4): 502-506, 2005

Assessment of morphology, antigenicity, and nucleic acid integrity for diagnostic thyroid pathology using formalin substitute fixatives. Thyroid 19(11): 1239-1248, 2010

Comparison of formalin-free tissue fixatives: a proteomic study testing their application for routine pathology and research. Archives of Pathology and Laboratory Medicine 135(6): 744-752, 2011

Evaluation of ethanol-based fixatives as a substitute for formalin in diagnostic clinical laboratories. Journal of Histotechnology 23(4): 299-308, December, 2000

The use of fixatives in diagnostic pathology. Journal of Histotechnology 24(3): 151-152, September, 2001

Leaching of glycosamino glycans from tissues by the fixatives formalin saline and formalin cetrimide. Histochemical Journal 7(4): 361-366, 1975

Synthetic production of fixatives simulating formalin-bromide and Cajal's formalin-uranium nitrate. Rev Asoc Med Argentina 61(609/610): 516-519, 1947

Experiences with reduced concentration of formalin in fixatives and formalin free conservation of gross anatomical specimens for dissection and demonstration. Anatomia Histologia Embryologia 16(3): 250-253, 1987

Systematic comparison of tissue fixation with alternative fixatives to conventional tissue fixation with buffered formalin in a xenograft-based model. Virchows Archiv 461(3): 259-269, 2012

Tissue transfer to pathology labs: under vacuum is the safe alternative to formalin. Virchows Archiv 452(2): 229-231, 2007

Staining reactions of mucopolysaccharides after formalin-containing fixatives. Stain Technology 38: 56-59, 1963

Current concepts in diagnostic histo pathology immuno histologic methods and their application in diagnostic histo pathology. Journal of Histotechnology 5(1): 35-39, 1982

Effects of formalin, methacarn, and fineFIX fixatives on RNA preservation. Diagnostic Molecular Pathology 19(2): 112-122, 2010

Forensic toxicological analyses of drugs in tissues in formalin solutions and in fixatives. Forensic Science International 249: 165-172, 2016

Post mortem inflation of lungs with formalin vapour: application to veterinary pathology. Veterinary Record 100(1): 11-11, 1977