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Application of real-time reverse transcription polymerase chain reaction for the detection of SVDV



Application of real-time reverse transcription polymerase chain reaction for the detection of SVDV



Polish Journal of Veterinary Sciences 12(1): 119-121



Application of real-time RT-PCR (rRT-PCR) for detection of swine vesicular disease virus (SVDV) in samples of archival SVDV isolates and clinical samples collected from SVDV infected pigs was described. A primer set that targets the IRES region of the SVDV genome and TaqMan probe specific for a highly conserved region in SVDV RNA IRES region were used. The assay detected viral RNA in all tested archival strains of SVDV isolated in Europe during years 1972-73 and 1992 as well as in clinical samples collected from experimentally infected pigs. The rRT-PCR can provide quantitative and qualitative information and is more sensitive and faster to perform than the conventional RT-PCR.

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Accession: 051612659

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PMID: 19459449


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